02048naa a2200241 a 450000100080000000500110000800800410001910000180006024501070007826000090018552013670019465000200156165000180158165300170159965300200161670000250163670000190166170000190168070000170169970000180171670000220173477300500175620331542024-06-20       2015    bl uuuu     u00u1 u    #d1 aFREIRE, M. S.  aNanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4.h[electronic resource]  c2015  aBone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells.  abone resorption  ainterleukin-4  aNanoUPLC-MSE  aOsteoimmunology1 aCANTUÁRIA, A. P. C.1 aLIMA, S. M. F.1 aALMEIDA, J. A.1 aMURAD, A. M.1 aFRANCO, O. L.1 aREZENDE, T. M. B.  tJournal of Proteomicsgv. 131, p. 8-16, 2015.