01834naa a2200289 a 450000100080000000500110000800800410001902400410006010000160010124500820011726000090019952010220020865000230123065000100125365000110126365000120127465000200128665300150130665300130132165300140133470000280134870000190137670000250139570000200142070000260144077300780146620319922021-10-04       2015    bl uuuu     u00u1 u    #d7 a10.1590/S0100-204X20150012000012DOI1 aREIS, K. B.  aCharacterization of rue extract and its potential for controlling rice blast.  c2015  aThe objective of this work was to purify and standardize the extract of rue (Ruta graveolens) and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers furanocoumarins psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.  aMagnaporthe oryzae  aArroz  aArruda  aBrusone  aRuta Graveolens  aBergapteno  aControle  aPsoraleno1 aCORTÊS, M. V. de C. B.1 aMARTINS, F. S.1 aFILIPPI, M. C. C. de1 aPAULA, J. R. de1 aCONCEIÇÃO, E. C. da  tPesquisa Agropecuária Brasileiragv. 50, n. 12, p. 1121-1130, dez. 2015.