01660naa a2200325 a 450000100080000000500110000800800410001902400520006010000190011224501250013126000090025630000130026552007490027865000140102765000130104165000200105465000140107465000150108865000210110365000110112465000160113565300180115165300170116965300170118670000190120370000190122270000210124170000230126277300490128520254872021-04-06       2015    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.btre.2015.02.0022DOI1 aMARTINS, P. K.  aA simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.h[electronic resource]  c2015  ap. 41-44  aAbstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane  aBioenergy  aGenomics  aSetaria viridis  aSugarcane  aBioenergia  aCana de Açúcar  aGenoma  aMilho Verde  aC4 metabolism  aGreen millet  aModel system1 aRIBEIRO, A. P.1 aDIAS, B. B. A.1 aKOBAYASHI, A. K.1 aMOLINARI, H. B. C.  tBiotechnology Reportsgv. 6, p. 41-44, 2015.