01921nam a2200253 a 450000100080000000500110000800800410001910000260006024501510008626001750023752010560041265000130146865000100148165000120149165000130150365300090151665300390152565300140156465300140157870000190159270000190161170000200163070000170165020239602021-09-06       2015    bl uuuu     u00u1 u    #d1 aBEZERRA JUNIOR, R. Q.  aMatrix metalloproteinases (MMPs) in buck blood chronically infected by arthritis encephalitis virus in Northeast of Brazil.h[electronic resource]  aIn: INTERNATIONAL CONGRESS ON ANALYTICAL PROTEOMICS, 4., Caparica. Book of abstracts; proceedings. Caparica: Universidade Nova de Lisboa, 2015. Abstract O8A. p. 83.c2015  aAbstract: The objective of this work was to investigate the activity of the MMPs (MMP-2 e MMP-9) in blood serum of chronically infected bucks by CAEV. The experiment was constituted by two groups (n = 5 each group). The first one was composed by five naturally infected bucks (4-5 years) and the second group constituted of five negative bucks (4-5 years) for CAE. Serology was performed using the Western Blotting (WB) and confirmed by Polymerase Chain Reaction (PCR). Blood samples were collected by puncturing the jugular vein from animals and evaluated by zymography (SDS-PAGE) using gelatin as substratum. The gel densitometry analysis showed higher activity (P&lt0.05) of the pro-MMP-2 and MMP-2 in seropositive group (Fig. 1). The higher activity of the MMPs (MMP-2 and MMP-9) in infected group to CAE suggests a role of these proteases in the chronic inflammatory process in goats, once the key role of MMPs molecules in inflammation are involved in pathophysiological remodeling of the vascular wall, even in animals without apparent disease.  aGenomics  aGoats  aCaprino  aProtease  aCAEV  aCaprine arthritis encephalit virus  aGenômica  aProteases1 aELOY, A. M. X.1 aFURTADO, J. R.1 aPINHEIRO, R. R.1 aANDRIOLI, A.