02344naa a2200301 a 450000100080000000500110000800800410001910000220006024500770008226000090015950001140016852013350028265000270161765000230164465300280166765300300169565300190172565300200174465300340176470000200179870000200181870000190183870000270185770000260188470000220191070000200193277300900195220229392017-05-25       2015    bl uuuu     u00u1 u    #d1 aPEREIRA, G. da S.  aOptimal use of SSR markers for varietal identification of upland cotton.  c2015  aTítulo em português: Uso otimizado de marcadores SSR para identificação varietal de algodoeiro herbáceo.  aThe objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs. Among these varieties, the mean genetic distance was 0.387 (minimum of 0 and maximum of 0.786). The dendrograms generated by the unweighted pair group method with arithmetic average (UPGMA) did not reflect the regions of Brazil (20 genotypes) or around the world (96 genotypes), where the varieties or lines were selected. Bootstrap resampling shows that genotype identification is viable with 19 loci. The polymorphic markers evaluated are useful to perform varietal identification in a large panel of cotton varieties and may be applied in studies of the species diversity.  aMicrosatellite repeats  aGossypium Hirsutum  aCultivar discrimination  aIntraspecific polymorhism  aMicrosatellite  aMicrossatélite  aPolimorfismo intraespecífico1 aCAZÉ, A. L. R.1 aSILVA, M. G. da1 aALMEIDA, V. C.1 aMAGALHAES, F. O. da C.1 aSILVA FILHO, J. L. da1 aBARROSO, P. A. V.1 aHOFFMANN, L. V.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 50, n. 7, p. 571-581, jul. 2015.