02606naa a2200361 a 450000100080000000500110000800800410001902400350006010000200009524501530011526000090026850000350027752015170031265000130182965000170184265000180185965000180187765000210189565000140191665000230193065000110195365000100196465000160197465300190199065300260200965300190203570000190205470000180207370000170209170000180210870000270212677300910215320187572016-03-18       2015    bl uuuu     u00u1 u    #d7 a10.1007/s00253-015-6620-32DOI1 aMASCARIN, G. M.  aGlucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores.  c2015  aPublicado online: 07 May 2015.  aThe filamentous fungus Beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. In this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of B. bassiana, ESALQ1432 and GHA. Results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentrations enhanced blastospore yields by both isolates. High glucose concentrations increased the osmotic pressure of the media and coincided with higher dissolved oxygen levels and increased production of significantly smaller blastospores compared with blastospores produced in media with lower concentrations of glucose. The desiccation tolerance of blastospores dried to less than 2.6 % moisture was not affected by the glucose concentration of the medium but was isolate dependent. Blastospores of isolate ESALQ1432 produced in media containing 140 g glucose L-1 showed greater virulence toward whitefly nymphs (Bemisia tabaci) as compared with blastospores produced in media containing 40 g glucose L-1. These results suggest a synergistic effect between glucose concentration and oxygen availability on changing morphology and enhancing the yield and efficacy of blastospores of B. bassiana, thereby facilitating the development of a costeffective production method for this blastospore-based bioinsecticide.  aAeration  aBlastospores  aFungal spores  aMorphogenesis  aMycoinsecticides  aVirulence  aBeauveria bassiana  aEsporo  aFungo  aMorfogenese  aBemicia tabaci  aDesiccation tolerance  aOsmoadaptation1 aJACKSON, M. A.1 aKOBORI, N. N.1 aBEHLE, R. W.1 aDUNLAP, C. A.1 aDELALIBERA JÚNIOR, I.  tApplied Microbiology and Biotechnology, Berlingv. 99, n. 16, p. 6653-6665, Aug. 2015.