01729naa a2200253 a 450000100080000000500110000800800410001910000210006024501300008126000090021150001590022052007890037965000280116865000110119665000280120765000130123565000150124865000230126365000230128665300210130965300340133070000210136477300900138511017972019-02-25       2000    bl uuuu     u00u1 u    #d1 aKOBAYASHI, A. K.  aEstablishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum  c2000  aTítulo em português: Estabelecimento de sisterna in vitro para estudos da resistência induzida à Xanthomonas campestris pv. malvacearum em algodoeiro.  aAn in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mg/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated hypersensitive response.  aCell suspension culture  acotton  aHypersensitive response  aAlgodão  aBacteriose  aCultura de Célula  aGossypium Hirsutum  aBacterial blight  aExtracellular polysaccharides1 aVIEIRA, L. G. E.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 35, n. 4, p. 719-725, abr. 2000.