01753naa a2200205 a 450000100080000000500110000800800410001902200140006010000180007424500950009226000090018752011490019665000110134570000200135670000220137670000260139870000220142470000250144677300760147120159852023-05-18       2015    bl uuuu     u00u1 u    #d  a1684-53151 aSOARES, T. L.  aEffect of collection time on the viability of banana pollen grains.h[electronic resource]  c2015  aThe objective of this study was to assess the pollen viability of six improve ddiploid banana plants (AA) collected in different periods of the day , in two seasons of the year (winter and summer), usingin vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10am, 12pm, 2pm and 4pm were evaluated . We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%KNO3, 0.03%Ca(NO3)2.4H2O, and 0.02%MgSO4.7H2O, solidified with 0.8% agar, adjusted todifferent pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride(TTC).The highest pollen germination rates and viability were obtained at 8amand the lowest at 4pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer,the pollen viability and in vitrogermination rates werehighest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollencollection time for use in cross breedingprograms for plant improvemen.  aBanana1 aSOUZA, E. H. de1 aSAMPAIO, L. F. S.1 aCOSTA, M. A. P. de C.1 aSILVA, S. de O. e1 aSEREJO, J. A. dos S.  tAfrican Journal of Biotechnologygv.14, n. 4, p.1207-1214, April, 2015.