01786naa a2200277 a 450000100080000000500110000800800410001910000190006024501060007926000090018552009690019465000150116365000180117865000210119665000410121765000110125865000160126965000130128565300330129865300130133165300140134470000180135870000280137670000200140477300840142420153362017-03-14       2015    bl uuuu     u00u1 u    #d1 aMARTINS, C. M.  aRhizobial diversity from stem and root nodules of Discolobiumand Aeschynomene.h[electronic resource]  c2015  aAmong the genera that exhibit stem nodulation, Aeschynomeneand Discolobiumare found in the Pantanal Mato-grossense (wetlands in the state ofMato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllumand A. fluminensiswere collected from various locations in the Pantanal de Poconésub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobiumand A. fluminensis were observed. Among the isolates obtained from D. pulchellum,there was a group that was not similar to any of the reference strains used, and most of the isolates analyzed by PCR-RFLP were similar to the genus Bradyrhizobium.  anodulation  aplant anatomy  aAnatomia vegetal  aFixação simbiótica de nitrogênio  aLegume  aNodulação  aSimbiose  aBiological nitrogen fixation  aSymbiose  aVegetable1 aBORGES, W. L.1 aCOSTA JÚNIOR, J. de S.1 aRUMJANEK, N. G.  tActa Scientiarum. Agronomy, Maringágv. 37, n. 2, p. 163-170, Apr./June, 2015.