02015naa a2200229 a 450000100080000000500110000800800410001910000210006024501360008126000090021752013250022665000140155165000180156565000100158365000180159365000130161170000210162470000250164570000230167070000290169377300630172220125802017-05-23       2015    bl uuuu     u00u1 u    #d1 aGONCALVES, R. M.  aDigitaria horizontalis and D. insularis as alternative hosts for Pantoea ananatis in brazilian maize fields.h[electronic resource]  c2015  aMaize white spot (MWS) caused by Pantoea ananatis is one of the most important foliar diseases of maize in Brazil. The limited knowledge concerning the dispersal, survival and the occurrence of alternative hosts for the pathogen has compromised the development of disease control strategies. The purpose of this study was to evaluate two weed species, Digitaria horizontalis and D. insularis, as alternative hosts for P. ananatis. The bacterial isolate Pa1DI isolated from MWS-like lesions in D. insularis and isolates Pa2ZM and WT-2 from maize MWS lesions were used for cross-inoculation tests in the greenhouse. The presumptive identity of all isolates was determined by Gram staining (?), indole production (+), adonitol fermentation test (?), ice nucleation activity (INA+), and 16S ribosomal DNA sequencing. Further characterization using species-specific primers for P. ananatis followed by nucleotide sequencing of PCR amplicons confirmed the identity of the isolates. MWS symptoms in the cross inoculation experiment were observed in maize and the two Digitaria species. Thus, these two weed species may act as alternative hosts for P. ananatis. These results that show D. horizontalis and D. insularis as possible P. ananatis reservoirs should be considered in the development of MWS disease control strategies.  aLeaf spot  aPhaeosphaeria  aWeeds  aMancha foliar  aZea mays1 aMEIRELLES, W. F.1 aFIGUEIREDO, J. E. F.1 aBALBI-PEÑA, M. I.1 aPACCOLA-MEIRELLES, L. D.  tJournal of Plant Pathologygv. 97, n. 1, p. 177-181, 2015.