01969naa a2200217 a 450000100080000000500110000800800410001902400360006010000170009624502030011326000090031652012400032565000190156565000140158465000200159870000230161870000170164170000220165870000200168077300510170020124922015-04-09       2014    bl uuuu     u00u1 u    #d7 a10.1007/s10532-014-9691-4.2DOI1 aRAMOS, D. T.  aAssessment of microbial communities associated with fermentative-methanogenic biodegradation of aromatic hydrocarbons in groundwater contaminated with a biodiesel blend (B20).h[electronic resource]  c2014  aA controlled field experiment was conducted to assess the potential for fermentative-methanogenic biostimulation (by ammonium-acetate injection) to enhance biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) as well as polycyclic aromatic hydrocarbons (PAHs) in groundwater contaminated with biodiesel B20 (20:80 v/v soybean biodiesel and diesel). Changes in microbial community structure were assessed by pyrosequencing 16S rRNA analyses. BTEX and PAH removal began 0.7 year following the release, concomitantly with the increase in the relative abundance of Desulfitobacterium and Geobacter spp. (from 5 to 52.7 % and 15.8 to 37.3 % of total Bacteria 16S rRNA, respectively), which are known to anaerobically degrade hydrocarbons. The accumulation of anaerobic metabolites acetate and hydrogen that could hinder the thermodynamic feasibility of BTEX and PAH biotransformations under fermentative/methanogenic conditions was apparently alleviated by the growing predominance of Methanosarcina. This suggests the importance of microbial population shifts that enrich microorganisms capable of interacting syntrophically to enhance the feasibility of fermentative-methanogenic bioremediation of biodiesel blend releases.  aBiodegradation  abiodiesel  aBiodegradação1 aSILVA, M. L. B. da1 aNOSSA, C. W.1 aALVAREZ, P. J. J.1 aCORSEUIL, H. X.  tBiodegradationgv. 25, n. 5, p. 681-691, 2014.