02433naa a2200337 a 450000100080000000500110000800800410001910000200006024501180008026000090019852014650020765000280167265000210170065000100172165000170173165000100174865000120175865000290177065000240179965000170182365000110184065300150185170000190186670000190188570000160190470000190192070000170193970000270195670000220198377300900200520100712015-05-25       2014    bl uuuu     u00u1 u    #d1 aPINTO, P. H. N.  aArtificial insemination in Cape Verdean Goats with cooled semen stored for 24 or 48 Hours.h[electronic resource]  c2014  aAbstract: The fertilizing capacity of goat semen stored at 5oC was evaluated in Cape Verdean goats. The semen was collected from three Canarian bucks and was diluted in Tris-egg yolk 2.5% extender to yield a final concentration of 150x106 mobile spermatozoa per 0.25 mL straw. The straws were inserted into a container and were maintained at 5oC for 24 (T24) or 48 hours (T48). A total of 133 goats were randomly assigned to receive one type of cooled semen. Estrus was synchronized with intravaginal sponges containing 60 mg of MAP for six days, plus 37.5 µg of d-cloprostenol and 200 IU of eCG, both of which were injected 24 h before sponge removal. Motility, strength, and HOST-reacted spermatozoa were recorded after cooling, according to treatment. Timed artificial insemination was performed at approximately 37 hours after sponge removal. Seminal motility (58.8±11.1 and 51.3±2.5%) and strength (2.9±0.5 and 2.8±0.3) did not differ (P&gt0.05) between the T24 and T48 samples. Swelled spermatozoa were superior (P&lt0.001) at T24 (63.5±19.7%) compared to T48 (31.2±22.1%). The pregnancy rates were similar (P&gt0.05) at T24 (26.5%) and T48 (21.5%). Goat semen maintains acceptable quality after cooling at 5ºC for 24 and 48 hours and can be used in artificial insemination programs in Cape Verde goats. Certain adjustments, including the time of artificial insemination relative to sponge removal, must be made to achieve better pregnancy rates.  aArtificial insemination  aCryopreservation  aGoats  aReproduction  aCabra  aCaprino  aInseminação artificial  aReprodução animal  aResfriamento  aSêmen  aCabo Verde1 aFREITAS, J. A.1 aFONSECA, J. D.1 aPILE, E. A.1 aESTEVES, L. V.1 aSOUZA, V. L.1 aSOUZA-FABJAN, J. M. G.1 aFONSECA, J. F. da  tAustralian Journal of Basic and Applied Sciencesgv. 8, n. 18, p. 473-478, Dec. 2014.