02123naa a2200205 a 450000100080000000500110000800800410001902200140006010000160007424501470009026000090023752015120024665000250175865000110178370000240179470000170181870000150183570000130185077300540186320096962023-05-26       2015    bl uuuu     u00u1 u    #d  a1664-302X1 aKÖBERL, M.  aAgroforestry leads to shifts within the gammaproteobacterial microbiome of banana plants cultivated in Central America.h[electronic resource]  c2015  aBananas(Musaspp.)belongtothemostimportantglobalfoodcommodities,andtheircultivationrepresentstheworld?slargestmonoculture.Althoughtheplant- associatedmicrobiomehassubstantialinfluenceonplantgrowthandhealth,thereisalackofknowledgeofthebananamicrobiomeanditsinfluencingfactors.Westudiedtheimpactof(i)biogeography,and(ii)agroforestryonthebanana-associatedgammaproteobacterialmicrobiomeanalyzingplantsgrowninsmallholderfarmsinNicaraguaandCostaRica.Profilesof16SrRNAgenesrevealedhighabundancesofPseudomonadales,Enterobacteriales,Xanthomonadales,andLegionellales.Anextraordinaryhighdiversityofthegammaproteobacterialmicrobiotawasobservedwithintheendophyticmicroenvironments(endorhizaandpseudostem),whichwassimilarinbothcountries.Enterobacteriawereidentifiedasdominantgroupofabove-groundplantparts(pseudostemandleaves).Neitherbiogeographynoragroforestryshowedastatisticallysignificantimpactonthegammaproteobacterialbananamicrobiomeingeneral.However,indicatorspeciesforeachmicroenvironmentandcountry,aswellasforplantsgrowninCoffeaintercroppingsystemswithandwithoutagri-silviculturalproductionofdifferentFabaceaetrees(Ingaspp.inNicaraguaandErythrinapoeppigianainCostaRica)couldbeidentified.Forexample,bananaplantsgrowninagroforestrysystemswerecharacterizedbyanincreaseofpotentialplant-beneficialbacteria,likePseudomonasandStenotrophomonas,andontheothersidebyadecreaseofErwinia.Hence,thisstudycouldshowthatasaresultoflegume-basedagroforestrytheindigenousbanana-associatedgammaproteobacterialcommunitynoticeablyshifted.  aGamma-Proteobacteria  aBanana1 aRODRIGUEZ, M. A. D.1 aMARTINUZ, A.1 aSTAVER, C.1 aBERG, G.  tFrontiers in microbiologygv. 6, Februrary, 2015.