02078naa a2200313 a 450000100080000000500110000800800410001910000230006024500830008326000090016652011710017565000160134665000180136265000150138065000240139565000270141965300260144665300230147270000230149570000260151870000190154470000210156370000220158470000170160670000260162370000160164970000250166577300740169020079372015-02-06       2015    bl uuuu     u00u1 u    #d1 aALBERTTI, L. A. G.  aMycobacteria species in wild mammals of the Pantanal of central South America.  c2015  aBovine tuberculosis is a chronic disease of economic importance in livestock, but may also infect wild animals and occasionally humans. Its principal infective agent is Mycobacterium bovis. The present study was Undertaken to examine the possible involvement of wildlife in the M. bovis life cycle in the Pantanal and to assess their potential role as a wild animal reservoir for this disease. DNA samples were obtained from 14 wild animals, namely 4 pampas deer (Ozotoceros bezoarticus), 9 feral pigs (Sus scrofa), and 1 coati (Nasua nasua).Aregion of the hsp65 gene, present in allmycobacteria, was amplified and seven species of Mycobacterium were identified. Mycobacterium avium was present in 50 % of the samples and in all three animal species. Unsurprisingly, several non-tuberculosis mycobacteria species, such as Mycobacterium saskatchewanense, Mycobacterium parafinicum, and Mycobacterium parascrofulaceum were also detected.One sample from a deer exhibited high sequence similarity to M. bovis reference strains, which was confirmed through analysis using a 24-locus MIRU-VNTR genotyping. The results highlight the need for targeted M. bovis surveillance.  aNasua nasua  aSouth America  aSus scrofa  aMycobacterium Bovis  aOzotoceros Bezoarticus  aTuberculosis Pantanal  aWildlife reservoir1 aSOUZA FILHO, A. F.1 aFONSECA JUNIOR, A. A.1 aFREITAS, M. E.1 aPELLEGRIN, A. O.1 aZIMMERMANN, N. P.1 aTOMAS, W. M.1 aPERES, I. A. H. F. S.1 aFONTANA, I.1 aOSÓRIO, A. L. A. R.  tEuropean Journal of Wildlife Researchgv. 61, n. 1, p. 163-166, 2015.