01869nam a2200301 a 450000100080000000500110000800800410001910000170006024500970007726001570017430000190033150000170035052009320036765000230129965000090132265300150133170000180134670000190136470000230138370000210140670000170142770000200144470000200146470000180148470000220150270000200152470000230154420061552020-01-22       2014    bl uuuu     u00u1 u    #d1 aSOUZA, M. M.  aMonoallelic expression of NNAT gene in Nelore steers skeletal muscle.h[electronic resource]  aIn: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10.; 2014, Vancouver. Proceedings... Vancouver: American Society of Animal Sciencec2014  aNão paginado.  aWCGALP 2014.  aABSTRACT: The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele expressed. These results demonstrate that NNAT may not have the paternal imprint conserved in adult bovine muscle, and it suggests a G allele-dependent expression.  aGenomic imprinting  aZebu  aNeuronatin1 aZERLOTINI, A.1 aTIZIOTO, P. C.1 aOLIVEIRA, P. S. N.1 aSOMAVILLA, A. L.1 aMOKRY, F. B.1 aCESAR, A. S. M.1 aDINIZ, W. J. S.1 aMUDADU, M. A.1 aNICIURA, S. C. M.1 aCOUTINHO, L. L.1 aREGITANO, L. C. A.