02064naa a2200349 a 450000100080000000500110000800800410001902400350006010000200009524501410011526000090025630000190026552010470028465000170133165000120134865000170136065000130137765000170139065000190140765000110142665000180143765000150145565000180147070000200148870000220150870000200153070000160155070000190156670000190158570000190160477300910162320040832017-09-20 2014 bl uuuu u00u1 u #d7 a10.1007/s10295-014-1533-12DOI1 aCOSTA, O. Y. A. aMicrobial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method.h[electronic resource] c2014 aNão paginado. aSugarcane ethanol production occurs in non-sterile conditions, and microbial contamination can decrease productivity. In this study, we assessed the microbial diversity of contaminants of ethanol production in an industrial facility in Brazil. Samples obtained at different stages were analyzed by pyrosequencing-based profiling of bacterial and archaeal 16S rRNA genes and the fungal internal transcribed spacer region. A total of 355 bacterial groups, 22 archaeal groups, and 203 fungal groups were identified, and community changes were related to temperature changes at certain stages. After fermentation, Lactobacillus and unclassified Lactobacillaceae accounted for nearly 100 % of the bacterial sequences. Predominant Fungi groups were ?unclassified Fungi,?Meyerozyma, and Candida. The predominant Archaea group was unclassified Thaumarchaeota. This is the first work to assess the diversity of Bacteria, and Archaea and Fungi associated with the industrial process of sugarcane-ethanol production using culture-independent techniques. abiodiversity aethanol afermentation aindustry amicrobiology aBiodiversidade aEtanol aFermentação aIndústria aMicrobiologia1 aSOUTO, B. de M.1 aTUPINAMBÁ, D. D.1 aBERGMANN, J. C.1 aKYAW, C. M.1 aKRUGGER, R. H.1 aBARRETO, C. C.1 aQUIRINO, B. F. tJournal of Industrial Microbiology & Biotechnology, Epub ahead of print, 18 Nov. 2014.