02094naa a2200253 a 450000100080000000500110000800800410001910000190006024501330007926000090021252013510022165000200157265000150159265000160160765000240162365300160164770000210166370000180168470000150170270000200171770000210173770000190175877300630177720023442022-10-05       2014    bl uuuu     u00u1 u    #d1 aBIANCHI, M. B.  aThe hematological profile of farmed Sorubim limabreference intervals, cell morphology and cytochemistry.h[electronic resource]  c2014  aHematology implies rapid and practical analysis to assist the diagnosis of fish homeostatic imbalance. This study determined the blood reference intervals in an important native South American catfish farmed in the Brazilian Pantanal wetland, Sorubim lima (Siluriformes, Pimelodidae), and describes the morphological and cytochemical characteristics of the cells. A total of 92 fish reared in a fish farm were examined for reference intervals 25 - 75% of total plasmatic protein, hemoglobin, red blood cell count (RBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and total thrombocyte and leukocyte count (WBC). Periodic acid Schiff (PAS) with salivaryamylase, bromophenol blue, Sudan black B and toluidine blue (metacromasy) were the cytochemical stains employed to signal glycogen, proteins, lipids and nuclei in thrombocytes and leucocytes. In the blood smears, monocytes, lymphocytes, neutrophils, basophils, eosinophils, PAS - positive granular lymphocytes (LG-PAS) and thrombocytes were observed. Siluriforme similar results were observed in neutrophils and eosinophils morphology. Compared to other pimelodidae RBC, hematocrit and MCV showed the lowest index variation. In contrast to other siluriformes fish, S. limashowed the concomitant presence of circulating eosinophils and basophils.  afreshwater fish  ahematology  aHematologia  aPeixe de água doce  aJurupensém1 aJERÔNIMO, G. T.1 aPÁDUA, S. B.1 aSATAKE, F.1 aISHIKAWA, M. M.1 aTAVARES-DIAS, M.1 aMARTINS, M. L.  tVeterinarski Arhiv, Zagrebgv. 84, n. 6, p. 677-690, 2014.