02209naa a2200241 a 450000100080000000500110000800800410001910000180006024502000007826000090027852013950028765000140168265300200169670000240171670000290174070000180176970000270178770000200181470000220183470000210185670000320187777300580190920011032022-04-05 2014 bl uuuu u00u1 u #d1 aAOYAGI, L. N. aGenomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi.h[electronic resource] c2014 aMyb genes constitute one of the largest transcription factor families in the plant kingdom. Soy-bean MYB transcription factors have been related to the plant response to biotic stresses. Their involvement in response to Phakopsora pachyrhizi infection has been reported by several transcrip-tional studies. Due to their apparently highly diverse functions, these genes are promising targets for developing crop varieties resistant to diseases. In the present study, the identi?cation and phy-logenetic analysis of the soybean R2R3-MYB (GmMYB) transcription factor family was performed and the expression pro?les of these genes under biotic stress were determined. GmMYBs were identi?ed from the soybean genome using bioinformatic tools, and their putative functions were determined based on the phylogenetic tree and classi?ed into subfamilies using guides AtMYBs describ-ing known functions. The transcriptional pro?les of GmMYBs upon infection with different pathogen were revealed by in vivo and in silico analyses. Selected target genes potentially involved in dis-ease responses were assessed by RT-qPCR after different times of inoculation with P. pachyrhizi using different genetic backgrounds related to resistance genes (Rpp2 and Rpp5). R2R3-MYB transcrip-tion factors related to lignin synthesis and genes responsive to chitin were signi?cantly induced in the resistant genotypes. aphylogeny aBiotic stresses1 aLOPES-CAITAR, V. S.1 aCARVALHO, M. C. C. G. de1 aDARBEN, L. M.1 aPOLIZEL-PODANOSQUI, A.1 aKUWAHARA, M. K.1 aNEPOMUCENO, A. L.1 aABDELNOOR, R. V.1 aMARCELINO-GUIMARÃES, F. C. tPlant Sciense, Limerickgv. 229, p. 32-42, Dec. 2014.