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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
29/01/1997 |
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Data da última atualização: |
07/05/2009 |
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Autoria: |
NOVAES, R. F. de F. |
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Título: |
Estudo comparativo da ciclagem de nutrientes em povoamentos puros e mistos de Pinus caribaea Mor. var hondurensis Bar. et Golf e Liquidambar styraciflua L. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
1987. |
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Páginas: |
145p. |
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Idioma: |
Português |
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Notas: |
Dissertação (Mestrado em Engenharia Florestal) - Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba. Orientador: Prof. Dr. Fabio Poggiani. |
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Palavras-Chave: |
Agudos; Ciclo; Cycling; Folhas; Nutrientes; São Paulo; Sarpler. |
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Thesagro: |
Liquidâmbar Styraciflua; Pinus Caribaea; Serapilheira. |
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Thesaurus Nal: |
leaves; nutrients. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00895nam a2200265 a 4500
001 1304796
005 2009-05-07
008 1987 bl uuuu m 00u1 u #d
100 1 $aNOVAES, R. F. de F.
245 $aEstudo comparativo da ciclagem de nutrientes em povoamentos puros e mistos de Pinus caribaea Mor. var hondurensis Bar. et Golf e Liquidambar styraciflua L.
260 $a1987.$c1987
300 $a145p.
500 $aDissertação (Mestrado em Engenharia Florestal) - Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba. Orientador: Prof. Dr. Fabio Poggiani.
650 $aleaves
650 $anutrients
650 $aLiquidâmbar Styraciflua
650 $aPinus Caribaea
650 $aSerapilheira
653 $aAgudos
653 $aCiclo
653 $aCycling
653 $aFolhas
653 $aNutrientes
653 $aSão Paulo
653 $aSarpler
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
22/05/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICKEL, O.; FAJARDO, T. V. M. |
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Afiliação: |
OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
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Título: |
Detection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Plant Pathology, v. 96, n. 1, p. 207-213, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs. |
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Palavras-Chave: |
RT-PCR. |
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Thesagro: |
DNA; Maça; Pera; Virus. |
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Thesaurus NAL: |
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple stem grooving virus; Apple stem pitting virus; Fruit quality; Reverse transcriptase polymerase chain reaction. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/986801/1/JPP2014RTPCRVirusmacieiraNickelThor.pdf
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Marc: |
LEADER 01818naa a2200265 a 4500
001 1986801
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aNICKEL, O.
245 $aDetection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes.$h[electronic resource]
260 $c2014
520 $aApple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs.
650 $aApple chlorotic leaf spot virus
650 $aApple mosaic virus
650 $aApple stem grooving virus
650 $aApple stem pitting virus
650 $aFruit quality
650 $aReverse transcriptase polymerase chain reaction
650 $aDNA
650 $aMaça
650 $aPera
650 $aVirus
653 $aRT-PCR
700 1 $aFAJARDO, T. V. M.
773 $tJournal of Plant Pathology$gv. 96, n. 1, p. 207-213, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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