| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Gado de Corte; Embrapa Meio-Norte; Embrapa Pecuária Sul; Embrapa Unidades Centrais. |
|
Data corrente: |
08/01/1991 |
|
Data da última atualização: |
25/01/2016 |
|
Autoria: |
MADRUGA, C. R.; HONER, M. R.; SCHENK, M. A. M.; CURVO, J. B. E. |
|
Afiliação: |
EMBRAPA. Centro Nacional de Pesquisa de Gado de Corte (Campo Grande, MS). |
|
Título: |
Avaliação preliminar de parâmetros epidemiológicos da tristeza parasitária bovina no Mato Grosso do Sul. |
|
Ano de publicação: |
1987 |
|
Fonte/Imprenta: |
Campo Grande, MS: EMBRAPA, CNPGC, 1987. |
|
Páginas: |
7 p. |
|
Série: |
(EMBRAPA-CNPGC. Pesquisa em Andamento, 38). |
|
Idioma: |
Português |
|
Notas: |
CNPGC. |
|
Conteúdo: |
Epidemiologia; Bovino; Tristeza parasitaária; Praga; Sanidade; América do Sul; Brasil; Mato Grosso do Sul.
|
|
Palavras-Chave: |
Avaliação; Babusia; Bovinae; Brasil; Mato Grosso do Sul; Parâmetro; Tristega parasitária; Tristeza; Tristeza parasitória. |
|
Thesagro: |
Bovino; Bovinocultura; Cerrado; Doença Animal; Epidemiologia; Parasito; Veterinária; Zootecnia. |
|
Thesaurus Nal: |
animal diseases; Brazil; epidemiology. |
|
Categoria do assunto: |
-- |
|
URL: |
https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/319331/1/PESQEMANDAMENTO38.pdf
|
|
Marc: |
LEADER 01221nam a2200421 a 4500
001 1319331
005 2016-01-25
008 1987 bl uuuu u0uu1 u #d
100 1 $aMADRUGA, C. R.
245 $aAvaliação preliminar de parâmetros epidemiológicos da tristeza parasitária bovina no Mato Grosso do Sul.
260 $aCampo Grande, MS: EMBRAPA, CNPGC$c1987
300 $a7 p.
490 $a(EMBRAPA-CNPGC. Pesquisa em Andamento, 38).
500 $aCNPGC.
520 $aEpidemiologia; Bovino; Tristeza parasitaária; Praga; Sanidade; América do Sul; Brasil; Mato Grosso do Sul.
650 $aanimal diseases
650 $aBrazil
650 $aepidemiology
650 $aBovino
650 $aBovinocultura
650 $aCerrado
650 $aDoença Animal
650 $aEpidemiologia
650 $aParasito
650 $aVeterinária
650 $aZootecnia
653 $aAvaliação
653 $aBabusia
653 $aBovinae
653 $aBrasil
653 $aMato Grosso do Sul
653 $aParâmetro
653 $aTristega parasitária
653 $aTristeza
653 $aTristeza parasitória
700 1 $aHONER, M. R.
700 1 $aSCHENK, M. A. M.
700 1 $aCURVO, J. B. E.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agricultura Digital. |
|
Data corrente: |
10/02/2014 |
|
Data da última atualização: |
08/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. |
|
Afiliação: |
MARINA DE MORAES MOURÃO, Centro de Pesquisas René Rachou-Fiocruz; MAINÁ BITAR, UFMG; FRANCISCO PEREIRA LOBO, CNPTIA; ANA PAULA PECONICK, Ufla; PRISCILA GRYNBERG, UFMG; FRANCISCO PROSDOCIMI, UFRJ; MICHAEL WAISBERG, University of Virginia; GUSTAVO COUTINHO CERQUEIRA, Broad Institute of Massachusetts Institute of Technology and Harvard; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; TIMOTHY YOSHINO, University of Wisconsin; GLÓRIA REGINA FRANCO, UFMG. |
|
Título: |
A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974). MenosSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different librari... Mostrar Tudo |
|
Palavras-Chave: |
Interferência de RNA. |
|
Thesagro: |
Schistosoma Mansoni. |
|
Thesaurus NAL: |
RNA interference; Trans-splicing. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02851naa a2200301 a 4500
001 1979292
005 2020-01-08
008 2013 bl uuuu u00u1 u #d
100 1 $aMOURÃO, M. de M.
245 $aA directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni.$h[electronic resource]
260 $c2013
520 $aSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
650 $aRNA interference
650 $aTrans-splicing
650 $aSchistosoma Mansoni
653 $aInterferência de RNA
700 1 $aBITAR, M.
700 1 $aLOBO, F. P.
700 1 $aPECONICK, A. P.
700 1 $aGRYNBERG, P.
700 1 $aPROSDOCIMI, F.
700 1 $aWAISBERG, M.
700 1 $aCERQUEIRA, G. C.
700 1 $aMACEDO, A. M.
700 1 $aMACHADO, C. R.
700 1 $aYOSHINO, T.
700 1 $aFRANCO, G. R.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 108, n. 6, p. 707-717, Sept. 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
