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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
06/12/2011 |
Data da última atualização: |
24/01/2020 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. |
Afiliação: |
MARINA MORAES MOURÃO, FIOCRUZ; MAINÁ LAST BITAR, UFMG, NUBIO; FRANCISCO PEREIRA LOBO, CNPTIA; PRISCILA GRYNBERG, UFMG; MARIANA BORONI, UFMG; MICHAEL WAISBERG, NIH; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; GLÓRIA REGINA FRANCO, UFMG. |
Título: |
Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. |
Páginas: |
Não paginado. |
Idioma: |
Inglês |
Notas: |
X-MEETING 2011. |
Conteúdo: |
Schistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG MenosSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstr... Mostrar Tudo |
Palavras-Chave: |
Expressão gênica. |
Thesagro: |
Schistosoma Mansoni. |
Thesaurus Nal: |
Gene expression. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/908752/1/Shistosoma.pdf
|
Marc: |
LEADER 02793nam a2200265 a 4500 001 1908752 005 2020-01-24 008 2011 bl uuuu u00u1 u #d 100 1 $aMOURÃO, M. M. 245 $aRevealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional$c2011 300 $aNão paginado. 500 $aX-MEETING 2011. 520 $aSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG 650 $aGene expression 650 $aSchistosoma Mansoni 653 $aExpressão gênica 700 1 $aBITAR, M. L. 700 1 $aLOBO, F. P. 700 1 $aGRYNBERG, P. 700 1 $aBORONI, M. 700 1 $aWAISBERG, M. 700 1 $aMACEDO, A. M. 700 1 $aMACHADO, C. R. 700 1 $aFRANCO, G. R.
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Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/04/2013 |
Data da última atualização: |
27/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
BARRETO, A. Q.; BRITO, S. X.; CARVALHO, C. A. L. de; NASCIMENTO, A. S. do; LEDO, C. A. da S. |
Afiliação: |
ANTÔNIO QUEIROZ BARRETO, Instituto de Meio Ambiente e Recursos Hídricos do Distrito Federal; SUELY XAVIER BRITO, ADAB; CARLOS ALFREDO LOPES DE CARVALHO, UFRB; ANTONIO SOUZA DO NASCIMENTO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
Título: |
Contact action of neem extract (Azadirachta indica A. Juss) on Tetragonisca angustula Latreille (Hymenoptera: Apidae) under controlled conditions. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Magistra, Cruz das Almas-BA, v. 24, número especial, p. 52-56, dez. 2012. |
ISSN: |
2236-4420 |
Idioma: |
Inglês |
Notas: |
Semana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012 |
Conteúdo: |
The neem extract is an alternative to the use of synthetic insecticides in agricultural pest control due to its repellent effect, by impact and by contact, in order to minimize damage to the environment. However, there is still a lack of studies on their beneficial effects on insects, especially stingless bees, as ?Jataí? bees (Tetragonisca angustula Latreille), widely distributed in Brazil and responsible for the pollination of many plant species of agricultural importance and forestry. Whereas toxicology studies and selectivity of insecticides on insect pollinators are becoming a major demand of agribusiness, with a view to increasing ecological awareness of society and environmental demands of consumers for safe food markets, this work aimed to evaluate the effect of the neem (Azadirachta indica A. Juss.) on workers of T. angustula. Neem extract showed the same level of toxicity that of the pyrethroid insecticide in commercial doses as a control, during the evaluation period of 24 hours. |
Palavras-Chave: |
Neem. |
Thesaurus NAL: |
stingless bees. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01776naa a2200217 a 4500 001 1956438 005 2023-06-27 008 2012 bl uuuu u00u1 u #d 022 $a2236-4420 100 1 $aBARRETO, A. Q. 245 $aContact action of neem extract (Azadirachta indica A. Juss) on Tetragonisca angustula Latreille (Hymenoptera$bApidae) under controlled conditions.$h[electronic resource] 260 $c2012 500 $aSemana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012 520 $aThe neem extract is an alternative to the use of synthetic insecticides in agricultural pest control due to its repellent effect, by impact and by contact, in order to minimize damage to the environment. However, there is still a lack of studies on their beneficial effects on insects, especially stingless bees, as ?Jataí? bees (Tetragonisca angustula Latreille), widely distributed in Brazil and responsible for the pollination of many plant species of agricultural importance and forestry. Whereas toxicology studies and selectivity of insecticides on insect pollinators are becoming a major demand of agribusiness, with a view to increasing ecological awareness of society and environmental demands of consumers for safe food markets, this work aimed to evaluate the effect of the neem (Azadirachta indica A. Juss.) on workers of T. angustula. Neem extract showed the same level of toxicity that of the pyrethroid insecticide in commercial doses as a control, during the evaluation period of 24 hours. 650 $astingless bees 653 $aNeem 700 1 $aBRITO, S. X. 700 1 $aCARVALHO, C. A. L. de 700 1 $aNASCIMENTO, A. S. do 700 1 $aLEDO, C. A. da S. 773 $tMagistra, Cruz das Almas-BA$gv. 24, número especial, p. 52-56, dez. 2012.
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