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| 3. |  | SHARMA, R. R.; SINGH, C. N.; CHHONKAR, O. P.; GOSWAMI, A. M.; SINGH, S. K. Polyphenol oxidase activity as an index for screening mango (Mangiferaindica L.) germplasm against malformation Plant Genetic Resources Newsletter, Rome, Italy, n.124, p.41-43, 2000| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | KROPF, W.; SINGH, S. K.; NARAYAN PRASAD, R. A.; SHARMA, O. P.; DE GROOT, B.; NIEUWHOF, G. A comparison of milk and reproductive performance of Sirohi goats with Alpine x Sirohi and Toggenburg x Sirohi crosses. In: INTERNATIONAL CONFERENCE ON GOATS, 5., 1992, New Delhi. Pre-conference proceedings abstracts contributory papers. New Delhi: International Goat Association, 1992. v. 1. p. 59.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/01/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VIEIRA, L. de J.; TAVARES FILHO, L. F. DE Q.; SOUZA, F. V. D.; ALVES, A. A. C.; OLIVEIRA, E. J. de. |
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Afiliação: |
L. DE J. VIEIRA, UFRB; L. F. DE Q. TAVARES FILHO, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ALFREDO AUGUSTO CUNHA ALVES, SRI; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development of interspecific hybrids of cassava and paternity analysis with molecular markers. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Agricultural Science, p. 1- 13, 2012. |
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ISSN: |
1469-5146 |
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DOI: |
10.1017/S0021859612000871 |
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Idioma: |
Inglês |
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Notas: |
Crops & Soils Research Paper. |
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Conteúdo: |
The present paper demonstrates the development of interspecific hybrids between Manihot esculenta Crantz ssp. esculenta (Mee) and M. esculenta Crantz ssp. flabellifolia (Mef) and paternity analysis using microsatellite markers [simple sequence repeat (SSR)]. Three Mef accessions (FLA005, FLA025V and FLA029V) were used for crosses with varieties of Mee: Saracura, Aipim Bravo, COL 1725, Aipim Rosa, Abóbora, Paraná and PER334. The paternity of the interspecific hybrids was investigated using 24 SSRs. The observed heterozygosity (Ho), polymorphic information content (PIC), probability of identity (PI) and paternity exclusion (PE) were evaluated. The rate of breeding success varied from 17 to 92%, and an average of two pollinations were required for each generated hybrid plant. The Ho value ranged from 0?11 to 0?92, and the PIC value ranged from 0?12 to 0?59. The uneven distribution of allele frequencies was accompanied by a high PI average (0?56). However, the combined PE for 21 loci was 0?99, which allows for the determination of the paternity of the hybrids with good discriminatory power. Of the 74 hybrids evaluated, 0?82 had their paternity confirmed using microsatellite markers. Discriminant analysis of principal components (DAPC) indicated the presence of eight clusters, of which, one was composed of only Mef varieties and the supposed hybrid Fla52Sar-H7, which was a product of apomixis. The parent Mee and hybrids were allocated in the other seven clusters. The data obtained demonstrate that SSR markers can be routinely used in breeding programmes to verify the paternity of interspecific crosses of cassava. MenosThe present paper demonstrates the development of interspecific hybrids between Manihot esculenta Crantz ssp. esculenta (Mee) and M. esculenta Crantz ssp. flabellifolia (Mef) and paternity analysis using microsatellite markers [simple sequence repeat (SSR)]. Three Mef accessions (FLA005, FLA025V and FLA029V) were used for crosses with varieties of Mee: Saracura, Aipim Bravo, COL 1725, Aipim Rosa, Abóbora, Paraná and PER334. The paternity of the interspecific hybrids was investigated using 24 SSRs. The observed heterozygosity (Ho), polymorphic information content (PIC), probability of identity (PI) and paternity exclusion (PE) were evaluated. The rate of breeding success varied from 17 to 92%, and an average of two pollinations were required for each generated hybrid plant. The Ho value ranged from 0?11 to 0?92, and the PIC value ranged from 0?12 to 0?59. The uneven distribution of allele frequencies was accompanied by a high PI average (0?56). However, the combined PE for 21 loci was 0?99, which allows for the determination of the paternity of the hybrids with good discriminatory power. Of the 74 hybrids evaluated, 0?82 had their paternity confirmed using microsatellite markers. Discriminant analysis of principal components (DAPC) indicated the presence of eight clusters, of which, one was composed of only Mef varieties and the supposed hybrid Fla52Sar-H7, which was a product of apomixis. The parent Mee and hybrids were allocated in the other seven clusters. The data obtaine... Mostrar Tudo |
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Palavras-Chave: |
Molecular markers. |
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Thesaurus NAL: |
cassava. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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001 1946814
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008 2012 bl uuuu u00u1 u #d
022 $a1469-5146
024 7 $a10.1017/S0021859612000871$2DOI
100 1 $aVIEIRA, L. de J.
245 $aDevelopment of interspecific hybrids of cassava and paternity analysis with molecular markers.$h[electronic resource]
260 $c2012
500 $aCrops & Soils Research Paper.
520 $aThe present paper demonstrates the development of interspecific hybrids between Manihot esculenta Crantz ssp. esculenta (Mee) and M. esculenta Crantz ssp. flabellifolia (Mef) and paternity analysis using microsatellite markers [simple sequence repeat (SSR)]. Three Mef accessions (FLA005, FLA025V and FLA029V) were used for crosses with varieties of Mee: Saracura, Aipim Bravo, COL 1725, Aipim Rosa, Abóbora, Paraná and PER334. The paternity of the interspecific hybrids was investigated using 24 SSRs. The observed heterozygosity (Ho), polymorphic information content (PIC), probability of identity (PI) and paternity exclusion (PE) were evaluated. The rate of breeding success varied from 17 to 92%, and an average of two pollinations were required for each generated hybrid plant. The Ho value ranged from 0?11 to 0?92, and the PIC value ranged from 0?12 to 0?59. The uneven distribution of allele frequencies was accompanied by a high PI average (0?56). However, the combined PE for 21 loci was 0?99, which allows for the determination of the paternity of the hybrids with good discriminatory power. Of the 74 hybrids evaluated, 0?82 had their paternity confirmed using microsatellite markers. Discriminant analysis of principal components (DAPC) indicated the presence of eight clusters, of which, one was composed of only Mef varieties and the supposed hybrid Fla52Sar-H7, which was a product of apomixis. The parent Mee and hybrids were allocated in the other seven clusters. The data obtained demonstrate that SSR markers can be routinely used in breeding programmes to verify the paternity of interspecific crosses of cassava.
650 $acassava
653 $aMolecular markers
700 1 $aTAVARES FILHO, L. F. DE Q.
700 1 $aSOUZA, F. V. D.
700 1 $aALVES, A. A. C.
700 1 $aOLIVEIRA, E. J. de
773 $tJournal of Agricultural Science, p. 1- 13, 2012.
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