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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
23/10/2014 |
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Data da última atualização: |
23/10/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PEREZ, E. A. P.; OLIVEIRA, C. G. R. de; FIGUEREDO, J. H. R.; SILVA, G. A. da; GIRARDI, C. L. |
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Afiliação: |
EDGARDO AQUILES PRADO PEREZ, CNPUV; CELSO GUARANI RUIZ DE OLIVEIRA, CNPUV; JOAO HENRIQUE RIBEIRO FIGUEREDO, CNPUV; GILDO ALMEIDA DA SILVA, CNPUV; CESAR LUIS GIRARDI, CNPUV. |
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Título: |
Planejamento e implantação do sistema de gestão da qualidade na Embrapa Uva e Vinho. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Fortaleza: Embrapa Agroindústria Tropical, 2014. p. 14. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho tem como objetivo propor uma abordagem para conduzir um processo de Implantação de um SGQ (Sistema de Gestão da Qualidade) e relatar os resultados recentes alcançados na Embrapa Uva e Vinho. |
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Palavras-Chave: |
Benchmarking; Embrapa Uva e Vinho; Gestão de conteúdo; Gestão de laboratórios; Sistema de Gestão da Qualidade; Sistema Embrapa de Qualidade. |
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Thesagro: |
Laboratório; Qualidade. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/998210/2/METEdgardo.pdf
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/998210/1/METEdgardo2.pdf
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Marc: |
LEADER 01218nam a2200253 a 4500
001 1998210
005 2014-10-23
008 2014 bl uuuu u00u1 u #d
100 1 $aPEREZ, E. A. P.
245 $aPlanejamento e implantação do sistema de gestão da qualidade na Embrapa Uva e Vinho.$h[electronic resource]
260 $aIn: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Fortaleza: Embrapa Agroindústria Tropical, 2014. p. 14.$c2014
520 $aEste trabalho tem como objetivo propor uma abordagem para conduzir um processo de Implantação de um SGQ (Sistema de Gestão da Qualidade) e relatar os resultados recentes alcançados na Embrapa Uva e Vinho.
650 $aLaboratório
650 $aQualidade
653 $aBenchmarking
653 $aEmbrapa Uva e Vinho
653 $aGestão de conteúdo
653 $aGestão de laboratórios
653 $aSistema de Gestão da Qualidade
653 $aSistema Embrapa de Qualidade
700 1 $aOLIVEIRA, C. G. R. de
700 1 $aFIGUEREDO, J. H. R.
700 1 $aSILVA, G. A. da
700 1 $aGIRARDI, C. L.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. |
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Afiliação: |
MARINA MORAES MOURÃO, FIOCRUZ; MAINÁ LAST BITAR, UFMG, NUBIO; FRANCISCO PEREIRA LOBO, CNPTIA; PRISCILA GRYNBERG, UFMG; MARIANA BORONI, UFMG; MICHAEL WAISBERG, NIH; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
X-MEETING 2011. |
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Conteúdo: |
Schistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG MenosSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstr... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus NAL: |
Gene expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/908752/1/Shistosoma.pdf
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Marc: |
LEADER 02793nam a2200265 a 4500
001 1908752
005 2020-01-24
008 2011 bl uuuu u00u1 u #d
100 1 $aMOURÃO, M. M.
245 $aRevealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional$c2011
300 $aNão paginado.
500 $aX-MEETING 2011.
520 $aSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG
650 $aGene expression
650 $aSchistosoma Mansoni
653 $aExpressão gênica
700 1 $aBITAR, M. L.
700 1 $aLOBO, F. P.
700 1 $aGRYNBERG, P.
700 1 $aBORONI, M.
700 1 $aWAISBERG, M.
700 1 $aMACEDO, A. M.
700 1 $aMACHADO, C. R.
700 1 $aFRANCO, G. R.
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Embrapa Agricultura Digital (CNPTIA) |
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