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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
03/03/2017 |
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Data da última atualização: |
04/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALDISSER, P. A. M. R.; PAPPAS JUNIOR, G. J.; MENEZES, I. P. P. de; MÜLLER, B. S. F.; PEREIRA, W. J.; NARCISO, M. G.; BRONDANI, C.; SOUZA, T. L. P. O.; BORBA, T. C. O.; VIANELLO, R. P. |
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Afiliação: |
PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; GEORGIOS J. PAPPAS JUNIOR, UNB; IVANDILSON P. P. DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; BARBARA S. F. MULLER, UNB; WENDELL J. PEREIRA, UFG; MARCELO GONCALVES NARCISO, CNPAF; CLAUDIO BRONDANI, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
SNP discovery in common bean by restriction-associated DNA (RAD) sequencing for genetic diversity and population structure analysis. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 291, n. 3, p. 1277-1291, June 2016. |
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ISSN: |
1617-4623 |
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DOI: |
10.1007/s00438-016-1182-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Researchers have made great advances into the development and application of genomic approaches for common beans, creating opportunities to driving more real and applicable strategies for sustainable management of the genetic resource towards plant breeding. This work provides useful polymorphic single-nucleotide polymorphisms (SNPs) for high-throughput common bean genotyping developed by RAD (restriction site-associated DNA) sequencing. The RAD tags were generated from DNA pooled from 12 common bean genotypes, including breeding lines of different gene pools and market classes. The aligned sequences identified 23,748 putative RAD-SNPs, of which 3357 were adequate for genotyping; 1032 RADSNPs with the highest ADT (assay design tool) score are presented in this article. The RAD-SNPs were structurally annotated in different coding (47.00 %) and non-coding (53.00 %) sequence components of genes. A subset of 384 RAD-SNPs with broad genome distribution was used to genotype a diverse panel of 95 common bean germplasms and revealed a successful amplification rate of 96.6 %, showing 73 % of polymorphic SNPs within the Andean group and 83 % in the Mesoamerican group. A slightly increased He (0.161, n = 21) value was estimated for the Andean gene pool, compared to the Mesoamerican group (0.156, n = 74). For the linkage disequilibrium (LD) analysis, from a group of 580 SNPs (289 RAD-SNPs and 291 BARC-SNPs) genotyped for the same set of genotypes, 70.2 % were in LD, decreasing to 0.10 %in the Andean group and 0.77 % in the Mesoamerican group. Haplotype patterns spanning 310 Mb of the genome (60 %) were characterized in samples from different origins. However, the haplotype frameworks were under-represented for the Andean (7.85 %) and Mesoamerican (5.55 %) gene pools separately. In conclusion, RAD sequencing allowed the discovery of hundreds of useful SNPs for broad genetic analysis of common bean germplasm. From now, this approach provides an excellent panel of molecular tools for whole genome analysis, allowing integrating and better exploring the common bean breeding practices. MenosResearchers have made great advances into the development and application of genomic approaches for common beans, creating opportunities to driving more real and applicable strategies for sustainable management of the genetic resource towards plant breeding. This work provides useful polymorphic single-nucleotide polymorphisms (SNPs) for high-throughput common bean genotyping developed by RAD (restriction site-associated DNA) sequencing. The RAD tags were generated from DNA pooled from 12 common bean genotypes, including breeding lines of different gene pools and market classes. The aligned sequences identified 23,748 putative RAD-SNPs, of which 3357 were adequate for genotyping; 1032 RADSNPs with the highest ADT (assay design tool) score are presented in this article. The RAD-SNPs were structurally annotated in different coding (47.00 %) and non-coding (53.00 %) sequence components of genes. A subset of 384 RAD-SNPs with broad genome distribution was used to genotype a diverse panel of 95 common bean germplasms and revealed a successful amplification rate of 96.6 %, showing 73 % of polymorphic SNPs within the Andean group and 83 % in the Mesoamerican group. A slightly increased He (0.161, n = 21) value was estimated for the Andean gene pool, compared to the Mesoamerican group (0.156, n = 74). For the linkage disequilibrium (LD) analysis, from a group of 580 SNPs (289 RAD-SNPs and 291 BARC-SNPs) genotyped for the same set of genotypes, 70.2 % were in LD, decreasing to 0.10 %... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Phaseolus vulgaris. |
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Thesaurus Nal: |
Beans; Fabaceae; Genetic variation; Haplotypes; Linkage disequilibrium; Single nucleotide polymorphism. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03207naa a2200361 a 4500
001 2066055
005 2021-10-04
008 2016 bl uuuu u00u1 u #d
022 $a1617-4623
024 7 $a10.1007/s00438-016-1182-3$2DOI
100 1 $aVALDISSER, P. A. M. R.
245 $aSNP discovery in common bean by restriction-associated DNA (RAD) sequencing for genetic diversity and population structure analysis.$h[electronic resource]
260 $c2016
520 $aResearchers have made great advances into the development and application of genomic approaches for common beans, creating opportunities to driving more real and applicable strategies for sustainable management of the genetic resource towards plant breeding. This work provides useful polymorphic single-nucleotide polymorphisms (SNPs) for high-throughput common bean genotyping developed by RAD (restriction site-associated DNA) sequencing. The RAD tags were generated from DNA pooled from 12 common bean genotypes, including breeding lines of different gene pools and market classes. The aligned sequences identified 23,748 putative RAD-SNPs, of which 3357 were adequate for genotyping; 1032 RADSNPs with the highest ADT (assay design tool) score are presented in this article. The RAD-SNPs were structurally annotated in different coding (47.00 %) and non-coding (53.00 %) sequence components of genes. A subset of 384 RAD-SNPs with broad genome distribution was used to genotype a diverse panel of 95 common bean germplasms and revealed a successful amplification rate of 96.6 %, showing 73 % of polymorphic SNPs within the Andean group and 83 % in the Mesoamerican group. A slightly increased He (0.161, n = 21) value was estimated for the Andean gene pool, compared to the Mesoamerican group (0.156, n = 74). For the linkage disequilibrium (LD) analysis, from a group of 580 SNPs (289 RAD-SNPs and 291 BARC-SNPs) genotyped for the same set of genotypes, 70.2 % were in LD, decreasing to 0.10 %in the Andean group and 0.77 % in the Mesoamerican group. Haplotype patterns spanning 310 Mb of the genome (60 %) were characterized in samples from different origins. However, the haplotype frameworks were under-represented for the Andean (7.85 %) and Mesoamerican (5.55 %) gene pools separately. In conclusion, RAD sequencing allowed the discovery of hundreds of useful SNPs for broad genetic analysis of common bean germplasm. From now, this approach provides an excellent panel of molecular tools for whole genome analysis, allowing integrating and better exploring the common bean breeding practices.
650 $aBeans
650 $aFabaceae
650 $aGenetic variation
650 $aHaplotypes
650 $aLinkage disequilibrium
650 $aSingle nucleotide polymorphism
650 $aDNA
650 $aFeijão
650 $aPhaseolus vulgaris
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aMENEZES, I. P. P. de
700 1 $aMÜLLER, B. S. F.
700 1 $aPEREIRA, W. J.
700 1 $aNARCISO, M. G.
700 1 $aBRONDANI, C.
700 1 $aSOUZA, T. L. P. O.
700 1 $aBORBA, T. C. O.
700 1 $aVIANELLO, R. P.
773 $tMolecular Genetics and Genomics$gv. 291, n. 3, p. 1277-1291, June 2016.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Classificação |
Cutter |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
07/11/2011 |
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Data da última atualização: |
07/02/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVA, G. M. da; BORTOLIN, F.; JANTSCH, E. M.; UHDE, L. T.; JEZEWSKI, T. J.; COSTA, P. U. N. da; MACHADO, L. J.; SAMBORSKI, T. |
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Afiliação: |
GUSTAVO MARTINS DA SILVA, CPPSUL; Felipe Bortolin, Acadêmico de Graduação de Agronomia/UNIJUÍ bolsista Rede Leite e estagiário EMBRAPA; Etiane Maroski Jantsch, Acadêmica de Graduação de Medicina Veterinária/UNIJUÍ, bolsista Rede Leite e estagiário EMBRAPA; Leonir Terezinha Uhde, Professora do Departamento de Estudos Agrários/UNIJUÍ; Tiago José Jezewski, Engenheiro Agrônomo do Departamento de Estudos Agrários/UNIJUÍ; Pedro Urubatan Neto da Costa, Engenheiro agrônomo, Escritório Regional da EMATER de Ijuí; Lediomar José Machado, Técnico em Agropecuária. Escritório Municipal da EMATER de São Valério; Tarcisio Samborski, Professor do Instituto Federal Farroupilha, Campus Santo Augusto. |
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Título: |
Monitoramento dos atributos físico-químicos do solo e proposições de fertilização em área de Cynodon dactylon sobressemeado com forrageiras de inverno. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 21., 2011, Maceió. Inovações tecnólogicas e mercado consumidor: [anais...]. Brasília, DF: Associação Brasileira de Zootecnistas, 2011. |
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Idioma: |
Português |
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Palavras-Chave: |
Rede Leite; Sustentabilidade. |
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Thesagro: |
Agricultura Familiar; Forragem; Produção Leiteira. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/905079/1/ENEX047.pdf
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Marc: |
LEADER 00922nam a2200241 a 4500
001 1905079
005 2012-02-07
008 2011 bl uuuu u00u1 u #d
100 1 $aSILVA, G. M. da
245 $aMonitoramento dos atributos físico-químicos do solo e proposições de fertilização em área de Cynodon dactylon sobressemeado com forrageiras de inverno.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE ZOOTECNIA, 21., 2011, Maceió. Inovações tecnólogicas e mercado consumidor: [anais...]. Brasília, DF: Associação Brasileira de Zootecnistas$c2011
650 $aAgricultura Familiar
650 $aForragem
650 $aProdução Leiteira
653 $aRede Leite
653 $aSustentabilidade
700 1 $aBORTOLIN, F.
700 1 $aJANTSCH, E. M.
700 1 $aUHDE, L. T.
700 1 $aJEZEWSKI, T. J.
700 1 $aCOSTA, P. U. N. da
700 1 $aMACHADO, L. J.
700 1 $aSAMBORSKI, T.
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Embrapa Pecuária Sul (CPPSUL) |
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