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| Registros recuperados : 13 | |
| 3. |  | PEDROSO, M. T. M.; MOREIRA, L. R.; CARVALHO, H. M. G.; BRAGA, I. M. V. Cadeias produtivas e inovação da agropecuária brasileira: passado e presente. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 56., 2018, Campinas. Transformações recentes na agropecuária brasileira: desafios em gestão, inovação, sustentabilidade e inclusão social: anais. Brasília, DF: SOBER, 2018.| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | TOLEDO, L. V.; DIAS, T. A. B.; MOREIRA, L. R.; BUSTAMANTE, P. G. Renovação do consentimento prévio fundamentado para pesquisa no território indígena Krahô. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 207.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | MOREIRA, L. R.; OLIVEIRA, E. E. de; PALLINI, A.; SANTOS, A. P. dos; OLIVEIRA, H. G. de. Comportamento de forrageamento do ácaro vermelho do tomateiro mediado por odores de plantas de tomate. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 331, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | SILVEIRA, M. H. L. S.; SIQUEIRA, F. G. de; RAU, M.; SILVA, L. da; MOREIRA, L. R. de S.; FILHO, E. X. F.; ANDREAUS, J. Hydrolysis of sugarcane bagasse with enzyme preparations from Acrophialophora nainiana grown on different carbon sources. Biocatalysis and Biotransformation, v. 32, n. 1, p. 53-63, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010.| Biblioteca(s): Embrapa Soja. |
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| 10. | | MOREIRA, L. R. de S.; CAMPOS, M. de C.; SIQUEIRA, P. H. V. M. de; SILVA, L. P. da; RICART, C. A. O.; MARTINS, P. A.; QUEIROZ, R. M. L.; FERREIRA FILHO, E. X. Two beta-xylanases from Aspergillus terreus: characterization and influence of phenolic compounds on xylanase activity. Fungal Genetics and Biology, v. 60, p. 46-52, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FERREIRA FILHO, E. X.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, 2011. Disponível em:Acesso em: 1 mar. 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FERREIRA FILHO, E. X.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, 2011.| Biblioteca(s): Embrapa Soja. |
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| 13. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; REIS, A. M. dos; GATEHOUSE, J. A.; SA, M. F. G. de. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, n. 14, 2011.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
14/01/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
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Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
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Palavras-Chave: |
Asian rust. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
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Marc: |
LEADER 02325naa a2200265 a 4500
001 1873147
005 2018-04-17
008 2010 bl --- 0-- u #d
100 1 $aVASCONCELOS, E. A. R.
245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
260 $c2010
520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control.
653 $aAsian rust
700 1 $aSANTANA, C. G.
700 1 $aGODOY, C. V.
700 1 $aSILVA, M. S.
700 1 $aMOREIRA, L. R. S.
700 1 $aOLIVEIRA-NETO, OSMUNDO B.
700 1 $aPRICE, D.
700 1 $aFITCHES, E.
700 1 $aFILHO, E. X. F.
700 1 $aMEHTA, A.
700 1 $aGATEHOUSE, J. A.
700 1 $aGROSSI-DE-SA, M. F.
773 $tIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010.
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