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| 7. |  | BARROS, N. E. F. de; OLIVEIRA, E. M. M.; PEREIRA, C. de A.; BERTOLINI, A. C.; FINARDI FILHO, F. Seleção de primers RAPD informativos para caracterização molecular de acessos de mandioca (Manihot esculenta) . In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 569 p. p. 250.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
24/06/2010 |
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Data da última atualização: |
23/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MACIEL, T. E. F.; FREIRE, M. C. M.; ALMEIDA, A. M. de R.; OLIVEIRA, L. O. de. |
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Afiliação: |
TALLES EDUARDO FERREIRA MACIEL, UFV; MAÍRA CRISTINA MENEZES FREIRE, UFV; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSO; LUIZ ORLANDO DE OLIVEIRA, UFV. |
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Título: |
Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 33, n. 2, p. 354-358, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
B-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the -tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in P. pachyrhizi. The gene structures of Pp tubB and orthologous -tubulin genes of Melampsora lini and Uromyces viciae-fabae were highly conserved. The amino acid substitutions in -tubulin proteins associated with the onset of benzimidazole resistance in model organisms, especially at His6, Glu198 and Phe200, were absent from the predicted sequence of the P. pachyrhizi -tubulin protein. |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Fungo; Phakopsora pachyrhizi; Soja. |
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Thesaurus NAL: |
Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01832naa a2200217 a 4500
001 1856082
005 2025-05-23
008 2010 bl uuuu u00u1 u #d
100 1 $aMACIEL, T. E. F.
245 $aMolecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust.$h[electronic resource]
260 $c2010
520 $aB-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the -tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in P. pachyrhizi. The gene structures of Pp tubB and orthologous -tubulin genes of Melampsora lini and Uromyces viciae-fabae were highly conserved. The amino acid substitutions in -tubulin proteins associated with the onset of benzimidazole resistance in model organisms, especially at His6, Glu198 and Phe200, were absent from the predicted sequence of the P. pachyrhizi -tubulin protein.
650 $aSoybean rust
650 $aFungo
650 $aPhakopsora pachyrhizi
650 $aSoja
653 $aFerrugem asiática da soja
700 1 $aFREIRE, M. C. M.
700 1 $aALMEIDA, A. M. de R.
700 1 $aOLIVEIRA, L. O. de
773 $tGenetics and Molecular Biology$gv. 33, n. 2, p. 354-358, 2010.
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Embrapa Soja (CNPSO) |
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