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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/11/2021 |
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Data da última atualização: |
29/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. |
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Afiliação: |
CAROLINE ISSLER RODRIGUES, UEFS; DIEGO MOTA DA COSTA, UEFS; ACIDÁLIA CARINE VIEIRA SANTOS, UFBA; MARIA JOSÉ MOREIRA BATATINHA, UFBA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; MARIANA BORGES BOTURA, UEFS; CLAYTON QUEIROZ ALVES, UEFS; TALIANE LEILA SOARES, UEFS; HUGO NEVES BRANDÃO, UEFS. |
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Título: |
Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.285, 2020. |
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ISSN: |
1873-2550 |
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DOI: |
10.1016/j.vetpar.2020.109219 |
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Idioma: |
Inglês |
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Conteúdo: |
Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. MenosSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to i... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02931naa a2200265 a 4500
001 2136781
005 2021-11-29
008 2020 bl uuuu u00u1 u #d
022 $a1873-2550
024 7 $a10.1016/j.vetpar.2020.109219$2DOI
100 1 $aRODRIGUES, C. I.
245 $aAssessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.$h[electronic resource]
260 $c2020
520 $aSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive.
650 $aAbacaxi
700 1 $aCOSTA, D. M. da
700 1 $aSANTOS, A. C. V.
700 1 $aBATATINHA, M. J. M.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, E. H. de
700 1 $aBOTURA, M. B.
700 1 $aALVES, C. Q.
700 1 $aSOARES, T. L.
700 1 $aBRANDÃO, H. N.
773 $tVeterinary Parasitology$gv.285, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
22/04/2010 |
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Data da última atualização: |
17/02/2020 |
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Tipo da produção científica: |
Sistema de Produção |
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Autoria: |
GASPAROTTO, L.; PEREIRA, J. C. R. (ed.). |
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Afiliação: |
LUADIR GASPAROTTO, CPAA; JOSE CLERIO REZENDE PEREIRA, CPAA. |
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Título: |
Cultura da bananeira no Estado do Amazonas. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Manaus: Embrapa Amazônia Ocidental, 2009. |
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Páginas: |
67 p. |
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Série: |
(Embrapa Amazônia Ocidental. Sistemas de produção, 4). |
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ISSN: |
1679-8880 |
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Idioma: |
Português |
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Conteúdo: |
Clima. Escolha da área de plantio. Solo. Cultivares. Produção e obtenção de mudas. Sintomas de deficiência nutricional. Planejamento do bananal. Amostragem para análise de solo. Calagem. Adubação de plantio. Plantio e replantio. Irrigação. Práticas culturais. Doenças e métodos de controle. Pragas e métodos de controle. Colheita e pós-colheita. Manejo pós-colheita. Coeficientes técnicos. |
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Palavras-Chave: |
Cultivo. |
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Thesagro: |
Banana; Sistema de Produção. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/697273/1/SP4banana.pdf
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Marc: |
LEADER 00936nam a2200193 a 4500
001 1697273
005 2020-02-17
008 2009 bl uuuu 00u1 u #d
022 $a1679-8880
100 1 $aGASPAROTTO, L.
245 $aCultura da bananeira no Estado do Amazonas.
260 $aManaus: Embrapa Amazônia Ocidental$c2009
300 $a67 p.
490 $a(Embrapa Amazônia Ocidental. Sistemas de produção, 4).
520 $aClima. Escolha da área de plantio. Solo. Cultivares. Produção e obtenção de mudas. Sintomas de deficiência nutricional. Planejamento do bananal. Amostragem para análise de solo. Calagem. Adubação de plantio. Plantio e replantio. Irrigação. Práticas culturais. Doenças e métodos de controle. Pragas e métodos de controle. Colheita e pós-colheita. Manejo pós-colheita. Coeficientes técnicos.
650 $aBanana
650 $aSistema de Produção
653 $aCultivo
700 1 $aPEREIRA, J. C. R.
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Embrapa Amazônia Ocidental (CPAA) |
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