| |
|
|
| Registros recuperados : 1 | |
| 1. |  | KIRCHHOF, G.; ECKERT, B.; STOFFELS, M.; BALDANI, J. I.; REIS, V. M.; HARTMANN, A. Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. International Journal of Systematic and Evolutionary Microbiology, v. 51, p.157-168, 2001.| Biblioteca(s): Embrapa Agrobiologia. |
|   |
| Registros recuperados : 1 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
09/07/2001 |
|
Data da última atualização: |
09/07/2001 |
|
Autoria: |
KIRCHHOF, G.; ECKERT, B.; STOFFELS, M.; BALDANI, J. I.; REIS, V. M.; HARTMANN, A. |
|
Título: |
Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. |
|
Ano de publicação: |
2001 |
|
Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, v. 51, p.157-168, 2001. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The enrichment of nitrogen-fixing bacteria from the C4-fibre plants, Spartina pectinata. Miscanthus sinensis. Miscanthus sacchariflorus and Pennisetum purpureum. with nitrogen-free semi-solid media led to the isolation of Herbaspirillum-Iike strains among other diazotrophic bacteria. On the basis of physiological properties. phylogenetic analysis comparing 165 rDNA sequences and DNA-DNA hybridization experiments of chromosomal DNA the new isolates could be grouped together in a new species with the proposed name Herbaspirillum frisingense sp. nov. Morphological characteristics. such as cell size and shape. colony appearance. motility and flagellation are largely identical to the known species Herbaspirillum rubrisubalbicans and Herbaspirillum seropedicae. On the basis of utilization of adipate (-). N-acetyl- D-glucosamine (+). meso-erythritol (-). L-rhamnose (-) and meso-inositol (-) Herbaspirillum frisingense sp. nov. can be distinguished from other known Herbaspirillum spp. Nitrogen-fixing capability was examined by PCR amplification of the nifD gene and an acetylene reduction assay. and was found with all isolates tested. 165 rDNA sequence similarity to the other Herbaspirillum spp. is 98.5-99.1 %. In genomic DNA-DNA hybridization experiments Herbaspirillum frisingense sp. nov. forms a homogeneous group with 70-100 +-10% similarity. clearly distinct from Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans with 1-34% similarity. 165 rRNA-targeted oligonucleotide probes. specific for the whole genus Herbaspirillum and for three Herbaspirillum species were designed and are suitable for fluorescence ;n situ hybridization. The DNA G+C content of Herbaspirillum frisingense sp. nov. is 63+ - 2 mol%. in agreement with the values of 61-65% for the genus. PCR fingerprinting exhibits a consistent pattern for groups of strains isolated from the same plant. suggesting a low genomic diversity among bacteria inhabiting C4-gramineous plant tissues. Low genetic DNA diversity seems to be common between probable endophytic bacterial isolates of the same taxon. The type strain of Herbaspirillum frisingense sp. nov. is GSF30T (= DSM 13128T). MenosThe enrichment of nitrogen-fixing bacteria from the C4-fibre plants, Spartina pectinata. Miscanthus sinensis. Miscanthus sacchariflorus and Pennisetum purpureum. with nitrogen-free semi-solid media led to the isolation of Herbaspirillum-Iike strains among other diazotrophic bacteria. On the basis of physiological properties. phylogenetic analysis comparing 165 rDNA sequences and DNA-DNA hybridization experiments of chromosomal DNA the new isolates could be grouped together in a new species with the proposed name Herbaspirillum frisingense sp. nov. Morphological characteristics. such as cell size and shape. colony appearance. motility and flagellation are largely identical to the known species Herbaspirillum rubrisubalbicans and Herbaspirillum seropedicae. On the basis of utilization of adipate (-). N-acetyl- D-glucosamine (+). meso-erythritol (-). L-rhamnose (-) and meso-inositol (-) Herbaspirillum frisingense sp. nov. can be distinguished from other known Herbaspirillum spp. Nitrogen-fixing capability was examined by PCR amplification of the nifD gene and an acetylene reduction assay. and was found with all isolates tested. 165 rDNA sequence similarity to the other Herbaspirillum spp. is 98.5-99.1 %. In genomic DNA-DNA hybridization experiments Herbaspirillum frisingense sp. nov. forms a homogeneous group with 70-100 +-10% similarity. clearly distinct from Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans with 1-34% similarity. 165 rRNA-targeted oligonucleotide... Mostrar Tudo |
|
Palavras-Chave: |
Diazotrophic bacteria; Herbaspirillum spp; Miscanthus spp. |
|
Thesagro: |
Pennisetum Purpureum. |
|
Thesaurus NAL: |
Spartina pectinata. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02913naa a2200241 a 4500
001 1598640
005 2001-07-09
008 2001 bl uuuu u00u1 u #d
100 1 $aKIRCHHOF, G.
245 $aHerbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants.
260 $c2001
520 $aThe enrichment of nitrogen-fixing bacteria from the C4-fibre plants, Spartina pectinata. Miscanthus sinensis. Miscanthus sacchariflorus and Pennisetum purpureum. with nitrogen-free semi-solid media led to the isolation of Herbaspirillum-Iike strains among other diazotrophic bacteria. On the basis of physiological properties. phylogenetic analysis comparing 165 rDNA sequences and DNA-DNA hybridization experiments of chromosomal DNA the new isolates could be grouped together in a new species with the proposed name Herbaspirillum frisingense sp. nov. Morphological characteristics. such as cell size and shape. colony appearance. motility and flagellation are largely identical to the known species Herbaspirillum rubrisubalbicans and Herbaspirillum seropedicae. On the basis of utilization of adipate (-). N-acetyl- D-glucosamine (+). meso-erythritol (-). L-rhamnose (-) and meso-inositol (-) Herbaspirillum frisingense sp. nov. can be distinguished from other known Herbaspirillum spp. Nitrogen-fixing capability was examined by PCR amplification of the nifD gene and an acetylene reduction assay. and was found with all isolates tested. 165 rDNA sequence similarity to the other Herbaspirillum spp. is 98.5-99.1 %. In genomic DNA-DNA hybridization experiments Herbaspirillum frisingense sp. nov. forms a homogeneous group with 70-100 +-10% similarity. clearly distinct from Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans with 1-34% similarity. 165 rRNA-targeted oligonucleotide probes. specific for the whole genus Herbaspirillum and for three Herbaspirillum species were designed and are suitable for fluorescence ;n situ hybridization. The DNA G+C content of Herbaspirillum frisingense sp. nov. is 63+ - 2 mol%. in agreement with the values of 61-65% for the genus. PCR fingerprinting exhibits a consistent pattern for groups of strains isolated from the same plant. suggesting a low genomic diversity among bacteria inhabiting C4-gramineous plant tissues. Low genetic DNA diversity seems to be common between probable endophytic bacterial isolates of the same taxon. The type strain of Herbaspirillum frisingense sp. nov. is GSF30T (= DSM 13128T).
650 $aSpartina pectinata
650 $aPennisetum Purpureum
653 $aDiazotrophic bacteria
653 $aHerbaspirillum spp
653 $aMiscanthus spp
700 1 $aECKERT, B.
700 1 $aSTOFFELS, M.
700 1 $aBALDANI, J. I.
700 1 $aREIS, V. M.
700 1 $aHARTMANN, A.
773 $tInternational Journal of Systematic and Evolutionary Microbiology$gv. 51, p.157-168, 2001.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
