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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
18/06/1997 |
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Data da última atualização: |
18/06/1997 |
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Autoria: |
SOUSA, V. F. de; ANDRADE, C. de L. T. de; SOUSA, A. de P.; AGUIAR NETTO, A. de O. |
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Título: |
Redistribuicao de agua em solo de textura arenosa sob irrigacao por gotejamento. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENGENHARIA AGRICOLA, 21, 1992, Santa Maria. Anais... Santa Maria: Sociedade Brasileira de Engenharia Agricola/UFSM, 1992. v. 2, p. 963-97 |
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Idioma: |
Português |
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Conteúdo: |
Na regiao de tabuleiros litoraneos, onde predominam solos de textura arenosa e ventos fortes, sistemas de irrigacao localizada, principalmente gotejamento, sao os mais adaptados. Devido suas caracteristicas, esses sistemas de irrigacao devem ser avaliados, tanto na uniformidade de aplicacao quanto na distribuicao de agua no solo, para verificar seus efeitos nas perdas por percolacao profunda e no volume de solo explorado pelo sistema radicular das culturas. Em testes realizados com gotejamento no Campo Experimental da Embrapa/CNPAI, aplicando tres volumes de agua (10, 20 e 30 litros) sob gotejamento, com vazao de emissor de 41.h-1, verificou-se que, nos tres casos, o processo de redistribuicao de agua no solo foi semelhante, havendo uma predominancia de fluxode agua para baixo, proporcionando um formato alongado do bulbo, com reducao progressiva de volume de solo umido. A medida que aumentou o volume aplicado, o fluxo vertical cresceu sensivelmente, acentuando-se as perdas por percolacao profunda. A aplicacao de 20 litros proporcionou um volume de solo umido capaz de atender a abrangencia do sistema radicular da maioria das culturas, sem ocorrer perdas por percolacao abaixo de 80 cm. |
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Palavras-Chave: |
Bulbo umido. |
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Thesagro: |
Irrigação por Gotejamento; Umidade do Solo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01889naa a2200193 a 4500
001 1054437
005 1997-06-18
008 1992 bl --- 0-- u #d
100 1 $aSOUSA, V. F. de
245 $aRedistribuicao de agua em solo de textura arenosa sob irrigacao por gotejamento.
260 $c1992
520 $aNa regiao de tabuleiros litoraneos, onde predominam solos de textura arenosa e ventos fortes, sistemas de irrigacao localizada, principalmente gotejamento, sao os mais adaptados. Devido suas caracteristicas, esses sistemas de irrigacao devem ser avaliados, tanto na uniformidade de aplicacao quanto na distribuicao de agua no solo, para verificar seus efeitos nas perdas por percolacao profunda e no volume de solo explorado pelo sistema radicular das culturas. Em testes realizados com gotejamento no Campo Experimental da Embrapa/CNPAI, aplicando tres volumes de agua (10, 20 e 30 litros) sob gotejamento, com vazao de emissor de 41.h-1, verificou-se que, nos tres casos, o processo de redistribuicao de agua no solo foi semelhante, havendo uma predominancia de fluxode agua para baixo, proporcionando um formato alongado do bulbo, com reducao progressiva de volume de solo umido. A medida que aumentou o volume aplicado, o fluxo vertical cresceu sensivelmente, acentuando-se as perdas por percolacao profunda. A aplicacao de 20 litros proporcionou um volume de solo umido capaz de atender a abrangencia do sistema radicular da maioria das culturas, sem ocorrer perdas por percolacao abaixo de 80 cm.
650 $aIrrigação por Gotejamento
650 $aUmidade do Solo
653 $aBulbo umido
700 1 $aANDRADE, C. de L. T. de
700 1 $aSOUSA, A. de P.
700 1 $aAGUIAR NETTO, A. de O.
773 $tIn: CONGRESSO BRASILEIRO DE ENGENHARIA AGRICOLA, 21, 1992, Santa Maria. Anais... Santa Maria: Sociedade Brasileira de Engenharia Agricola/UFSM, 1992.$gv. 2, p. 963-97
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