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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/10/2018 |
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Data da última atualização: |
10/02/2025 |
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Autoria: |
OLIVEIRA, L. C.; FIGUEIREDO, J. R.; LIMA, A. K. F.; GONÇALVES, P. B. D.; BEZERRA, M. B. |
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Título: |
Desenvolvimento de um método enzimático para o isolamento de folículos ovarianos pré-antrais caprinos. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Ciência Animal, v. 7, n. 2, p. 116, jul./dez. 1997. |
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Idioma: |
Português |
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Notas: |
Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza. |
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Palavras-Chave: |
Folículo ovariano; Método enzimático. |
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Thesagro: |
Caprino; Ovário. |
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Thesaurus Nal: |
Goats; Ovarian follicles. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 00784nam a2200229 a 4500
001 2098230
005 2025-02-10
008 1997 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, L. C.
245 $aDesenvolvimento de um método enzimático para o isolamento de folículos ovarianos pré-antrais caprinos.
260 $aCiência Animal, v. 7, n. 2, p. 116, jul./dez. 1997.$c1997
500 $aEdição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.
650 $aGoats
650 $aOvarian follicles
650 $aCaprino
650 $aOvário
653 $aFolículo ovariano
653 $aMétodo enzimático
700 1 $aFIGUEIREDO, J. R.
700 1 $aLIMA, A. K. F.
700 1 $aGONÇALVES, P. B. D.
700 1 $aBEZERRA, M. B.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
15/08/2006 |
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Data da última atualização: |
28/10/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S.; CAMPO, R. J. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; RUBENS JOSE CAMPO, CNPSO. |
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Título: |
Genetic diversity of rhizobia associated with common bean (Phaseolus vulgaris L.) grown under no-tillage and conventional systems in Southern Brazil. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Applied Soil Ecology, v. 32, n. 2, p. 210-220, Jun. 2006. |
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DOI: |
10.1016/j.apsoil.2005.06.008 |
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Idioma: |
Inglês |
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Conteúdo: |
Brazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stability offered by the NT system has led to a decrease in the number of species, with the predominance of the most successful ones, although genetic diversity within each species has increased. The results obtained in this study show that we still understand poorly the relation between microbial diversity and soil sustainability and that the complexity of the ecosystems require the evaluation of several parameters to define and monitor soil quality. MenosBrazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stab... Mostrar Tudo |
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Palavras-Chave: |
Rizóbio. |
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Thesagro: |
Feijão; Fixação de Nitrogênio. |
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Thesaurus NAL: |
Beans; Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02686naa a2200229 a 4500
001 1469534
005 2024-10-28
008 2006 bl uuuu u00u1 u #d
024 7 $a10.1016/j.apsoil.2005.06.008$2DOI
100 1 $aKASCHUK, G.
245 $aGenetic diversity of rhizobia associated with common bean (Phaseolus vulgaris L.) grown under no-tillage and conventional systems in Southern Brazil.$h[electronic resource]
260 $c2006
520 $aBrazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stability offered by the NT system has led to a decrease in the number of species, with the predominance of the most successful ones, although genetic diversity within each species has increased. The results obtained in this study show that we still understand poorly the relation between microbial diversity and soil sustainability and that the complexity of the ecosystems require the evaluation of several parameters to define and monitor soil quality.
650 $aBeans
650 $aNitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aRizóbio
700 1 $aHUNGRIA, M.
700 1 $aANDRADE, D. S.
700 1 $aCAMPO, R. J.
773 $tApplied Soil Ecology$gv. 32, n. 2, p. 210-220, Jun. 2006.
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