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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
ABDELNOOR, R. V.; MORIYAMA, H.; MACKENZIE, S. |
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Título: |
Identification of Msh-1-homologue genes in soybean, rice, tomato and common bean. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 255. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The nuclear gene Msh1 (MutS homologue 1), recently cloned in Arabidopsis (Abdelnoor et al., 2003, PNAS 100:5968-5973), is involved in regulation of the copy number of subgenomic mitochondrial DNA molecules created by ectopic recombination in a process known as substoichiometric shifting. The Arabidopsis genome contains several other MutS homologues, but MSH1 protein is the only one that is targeted to both mitochondria and chloroplast and the predicted computer-based structure of this protein is very similar to the E. coli MUTS. We have isolated and sequenced the full-length homologous Msh1 genes in soybean, rice, tomato, and common bean to better understand their structure and function in plants. The tomato and soybean genes were obtained based on EST sequences identified by similarity to the Arabidopsis Msh1 gene and by RT-PCR of total RNA from leaves. The rice Msh1 was identified by searching the rice BAC sequences database and the coding sequence was confirmed by RT-PCR. The bean Msh1 was isolated from a BAC library, using a sequence of the soybean Msh1 gene as probe. Similar to Arabidopsis Msh1, all of them are comprised of 22 exons with very well conserved size and splicing position. Phylogenetic analysis using ClustalW showed that these genes have an overall identity of about 60% at the protein level. Based on computer prediction analysis they are predicted to be targeted to mitochondria. Additionally, several other ESTs have been identified in other plant species (barley, wheat, potato, tobacco, sorghum, maize and medicago), and show high homology to the sequenced Msh1 genes. The alignment of all of these MSH1 proteins has allowed us to identify specific conserved domains that might be very important for their proper function in plant mitochondria. MenosThe nuclear gene Msh1 (MutS homologue 1), recently cloned in Arabidopsis (Abdelnoor et al., 2003, PNAS 100:5968-5973), is involved in regulation of the copy number of subgenomic mitochondrial DNA molecules created by ectopic recombination in a process known as substoichiometric shifting. The Arabidopsis genome contains several other MutS homologues, but MSH1 protein is the only one that is targeted to both mitochondria and chloroplast and the predicted computer-based structure of this protein is very similar to the E. coli MUTS. We have isolated and sequenced the full-length homologous Msh1 genes in soybean, rice, tomato, and common bean to better understand their structure and function in plants. The tomato and soybean genes were obtained based on EST sequences identified by similarity to the Arabidopsis Msh1 gene and by RT-PCR of total RNA from leaves. The rice Msh1 was identified by searching the rice BAC sequences database and the coding sequence was confirmed by RT-PCR. The bean Msh1 was isolated from a BAC library, using a sequence of the soybean Msh1 gene as probe. Similar to Arabidopsis Msh1, all of them are comprised of 22 exons with very well conserved size and splicing position. Phylogenetic analysis using ClustalW showed that these genes have an overall identity of about 60% at the protein level. Based on computer prediction analysis they are predicted to be targeted to mitochondria. Additionally, several other ESTs have been identified in other plant species (ba... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02659naa a2200181 a 4500
001 1466830
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aABDELNOOR, R. V.
245 $aIdentification of Msh-1-homologue genes in soybean, rice, tomato and common bean.
260 $c2004
300 $ap. 255.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe nuclear gene Msh1 (MutS homologue 1), recently cloned in Arabidopsis (Abdelnoor et al., 2003, PNAS 100:5968-5973), is involved in regulation of the copy number of subgenomic mitochondrial DNA molecules created by ectopic recombination in a process known as substoichiometric shifting. The Arabidopsis genome contains several other MutS homologues, but MSH1 protein is the only one that is targeted to both mitochondria and chloroplast and the predicted computer-based structure of this protein is very similar to the E. coli MUTS. We have isolated and sequenced the full-length homologous Msh1 genes in soybean, rice, tomato, and common bean to better understand their structure and function in plants. The tomato and soybean genes were obtained based on EST sequences identified by similarity to the Arabidopsis Msh1 gene and by RT-PCR of total RNA from leaves. The rice Msh1 was identified by searching the rice BAC sequences database and the coding sequence was confirmed by RT-PCR. The bean Msh1 was isolated from a BAC library, using a sequence of the soybean Msh1 gene as probe. Similar to Arabidopsis Msh1, all of them are comprised of 22 exons with very well conserved size and splicing position. Phylogenetic analysis using ClustalW showed that these genes have an overall identity of about 60% at the protein level. Based on computer prediction analysis they are predicted to be targeted to mitochondria. Additionally, several other ESTs have been identified in other plant species (barley, wheat, potato, tobacco, sorghum, maize and medicago), and show high homology to the sequenced Msh1 genes. The alignment of all of these MSH1 proteins has allowed us to identify specific conserved domains that might be very important for their proper function in plant mitochondria.
700 1 $aMORIYAMA, H.
700 1 $aMACKENZIE, S.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
19/09/1997 |
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Data da última atualização: |
27/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HUNGRIA, M.; ANDRADE, D. de S.; COLOZZI-FILHO, A.; BALOTA, E. L. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ. |
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Título: |
Interação entre microrganismos do solo, feijoeiro e milho em monocultura ou consórcio. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 32, n. 8, p. 807-818, ago. 1997. |
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Idioma: |
Português |
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Conteúdo: |
Os sistemas de cultivo afetam a microbiota do solo e, neste estudo, procurou-se investigar algumas interações entre microrganismos de importância agrícola e o feijoeiro (Phaseolus vulgaris) e milho (Zea mays) em monocultura ou consórcio. Em condições controladas, tanto os exsudatos de feijão como os de milho estimularam a sobrevivência, o crescimento e a indução do genes nod de Rhizobium, a nodulação e o acúmulo de N no feijoeiro e a sobrevivência de Azospirillum lipoferum. Em um solo cultivado por dois anos com feijoeiro e milho em monocultura e consórcio, a população de Azospirillum spp. foi elevada e não diferiu entre os tratamentos, enquanto a população de Rhizobium tropici, os oxidantes do nitrito e a biomassa microbiana foram favorecidas pela presença do feijoeiro em consórcio ou monocultura. A espécie Rhizobium etli representou cerca de 15% da população rizobiana, exceto na monocultura de milho, em que esteve ausente e na qual predominou Rhizobium spp., capaz de nodular somente leucena (Leucaena leucocephala). Extratos de solo sob consórcio apresentaram teores mais elevados de compostos fenólicos indutores dos genes nod de Rhizobium. Os tratamentos não afetaram o número de esporos de fungos micorrízico-arbusculares, embora o feijoeiro em monocultura tenha proporcionado maior diversidade de espécies fúngicas. |
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Palavras-Chave: |
Bacteria diazotrofica; Biological nitrogen fixation; Biomassa microbiana; Diazotrophic bacteria; Fixacao biologica de nitrogenio; Fungo micorrizico; Microbial mass; Microrganismo amonificador; Microrganismo oxidante do nitrito; Nitrifiers. |
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Thesagro: |
Consorciação de Cultura; Feijão; Fixação de Nitrogênio; Milho; Phaseolus Vulgaris; Rhizobium; Zea Mays. |
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Thesaurus NAL: |
Azospirillum; Beans; Corn; Intercropping; mycorrhizal fungi; Nitrogen fixation. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/463640/1/ID-19648.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/44914/1/INTERACAO-ENTRE-MICRORGANISMOS-DO-SOLO.pdf
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Marc: |
LEADER 02623naa a2200433 a 4500
001 1463640
005 2025-03-27
008 1997 bl uuuu u00u1 u #d
100 1 $aHUNGRIA, M.
245 $aInteração entre microrganismos do solo, feijoeiro e milho em monocultura ou consórcio.
260 $c1997
520 $aOs sistemas de cultivo afetam a microbiota do solo e, neste estudo, procurou-se investigar algumas interações entre microrganismos de importância agrícola e o feijoeiro (Phaseolus vulgaris) e milho (Zea mays) em monocultura ou consórcio. Em condições controladas, tanto os exsudatos de feijão como os de milho estimularam a sobrevivência, o crescimento e a indução do genes nod de Rhizobium, a nodulação e o acúmulo de N no feijoeiro e a sobrevivência de Azospirillum lipoferum. Em um solo cultivado por dois anos com feijoeiro e milho em monocultura e consórcio, a população de Azospirillum spp. foi elevada e não diferiu entre os tratamentos, enquanto a população de Rhizobium tropici, os oxidantes do nitrito e a biomassa microbiana foram favorecidas pela presença do feijoeiro em consórcio ou monocultura. A espécie Rhizobium etli representou cerca de 15% da população rizobiana, exceto na monocultura de milho, em que esteve ausente e na qual predominou Rhizobium spp., capaz de nodular somente leucena (Leucaena leucocephala). Extratos de solo sob consórcio apresentaram teores mais elevados de compostos fenólicos indutores dos genes nod de Rhizobium. Os tratamentos não afetaram o número de esporos de fungos micorrízico-arbusculares, embora o feijoeiro em monocultura tenha proporcionado maior diversidade de espécies fúngicas.
650 $aAzospirillum
650 $aBeans
650 $aCorn
650 $aIntercropping
650 $amycorrhizal fungi
650 $aNitrogen fixation
650 $aConsorciação de Cultura
650 $aFeijão
650 $aFixação de Nitrogênio
650 $aMilho
650 $aPhaseolus Vulgaris
650 $aRhizobium
650 $aZea Mays
653 $aBacteria diazotrofica
653 $aBiological nitrogen fixation
653 $aBiomassa microbiana
653 $aDiazotrophic bacteria
653 $aFixacao biologica de nitrogenio
653 $aFungo micorrizico
653 $aMicrobial mass
653 $aMicrorganismo amonificador
653 $aMicrorganismo oxidante do nitrito
653 $aNitrifiers
700 1 $aANDRADE, D. de S.
700 1 $aCOLOZZI-FILHO, A.
700 1 $aBALOTA, E. L.
773 $tPesquisa Agropecuária Brasileira$gv. 32, n. 8, p. 807-818, ago. 1997.
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