| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
30/08/2018 |
|
Data da última atualização: |
30/08/2018 |
|
Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
|
Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
|
Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
|
Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
|
Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
|
Thesagro: |
Eqüino. |
|
Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
|
|
Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Algodão. |
|
Data corrente: |
22/02/2008 |
|
Data da última atualização: |
09/05/2008 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
FIRMINO, P. de T.; ALVES, S. de M.; BELTRÃO, N. E. de M.; BARROS, A. J. M.; SOUZA, A. G. de; SILVA, A. C.; ALVES, H. S.; ANJOS, G. G. dos; OLIVEIRA, D. M. |
|
Afiliação: |
Paulo de Tarso Firmino, Embrapa Algodão; Sérgio de Melo Alves, CPATU; Napoleão Esberard de Macedo Beltrão, Embrapa Algodão; Aldre Jorge Morais Barros, UFPB; Antônio Gouveia de Souza, UFPB; Ayice Chaves Silva, Embrapa Algodão; Hamilton Santos Alves, Estagiário; Gislayne Galdino dos Anjos, estagiária; Dalany Meneses Oliveira, estagiária. |
|
Título: |
Avaliação dos teores de óleo de algodão colorido BRS Rubi e Safira em relação ao algodão tradicional. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 4., 2007, Varginha, MG. Anais...Varginha: UFLA, 2007. |
|
Descrição Física: |
1 CD-ROM |
|
Idioma: |
Português |
|
Conteúdo: |
As cultivares de algodoeiro coloridas BRS Safira e BRS Rubi foram obtidas por
meio do cruzamento entre variedades de fibra branca e coloridas. Objetivou-se com essa
pesquisa estudar a composição em ácidos graxos nas sementes de algodoeiro Safira e Rubi, as quais foram analisadas por cromatografia gasosa. Como resultado observou-se que esta cultivar, tem maior concentração de lipídios nas sementes, 23,5%, comparada com as cultivares herbáceas convencionais, que contém média de 14%. No tocante aos ácidos graxos palmítico saturado, tem cerca do dobro do que possuem as cultivares convencionais e quanto aos ácidos graxos linoléico, tem média de 33,13%, enquanto nas sementes herbáceo tradicionais a média é de 47,8%. Já o teor de óleo da cultivar Rubi, é em média de 24,28%, possui os lipídeos da semente diferentes das cultivares brancas, acompanhando os teores da Safira, especialmente, no tocante aos ácidos graxos palmítico, saturado, que é cerca do dobro do normal nas cultivares de algodão comum. O ácido graxo linoléico, que é predominante no algodão branco, com 54,54% , já na Rubi, é bem mais baixo, com 31,88%. Os resultados demonstraram que as duas variedades apresentaram teores superiores constituintes acima da variedade de fibra branca. |
|
Palavras-Chave: |
Cultivares; Cultivares de algodão; Lipídios; Subproduto do algodoeiro; Subprodutos do algodoeiro. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02213naa a2200289 a 4500
001 1276917
005 2008-05-09
008 2007 bl uuuu u00u1 u #d
100 1 $aFIRMINO, P. de T.
245 $aAvaliação dos teores de óleo de algodão colorido BRS Rubi e Safira em relação ao algodão tradicional.
260 $c2007
300 $c1 CD-ROM
520 $aAs cultivares de algodoeiro coloridas BRS Safira e BRS Rubi foram obtidas por meio do cruzamento entre variedades de fibra branca e coloridas. Objetivou-se com essa pesquisa estudar a composição em ácidos graxos nas sementes de algodoeiro Safira e Rubi, as quais foram analisadas por cromatografia gasosa. Como resultado observou-se que esta cultivar, tem maior concentração de lipídios nas sementes, 23,5%, comparada com as cultivares herbáceas convencionais, que contém média de 14%. No tocante aos ácidos graxos palmítico saturado, tem cerca do dobro do que possuem as cultivares convencionais e quanto aos ácidos graxos linoléico, tem média de 33,13%, enquanto nas sementes herbáceo tradicionais a média é de 47,8%. Já o teor de óleo da cultivar Rubi, é em média de 24,28%, possui os lipídeos da semente diferentes das cultivares brancas, acompanhando os teores da Safira, especialmente, no tocante aos ácidos graxos palmítico, saturado, que é cerca do dobro do normal nas cultivares de algodão comum. O ácido graxo linoléico, que é predominante no algodão branco, com 54,54% , já na Rubi, é bem mais baixo, com 31,88%. Os resultados demonstraram que as duas variedades apresentaram teores superiores constituintes acima da variedade de fibra branca.
653 $aCultivares
653 $aCultivares de algodão
653 $aLipídios
653 $aSubproduto do algodoeiro
653 $aSubprodutos do algodoeiro
700 1 $aALVES, S. de M.
700 1 $aBELTRÃO, N. E. de M.
700 1 $aBARROS, A. J. M.
700 1 $aSOUZA, A. G. de
700 1 $aSILVA, A. C.
700 1 $aALVES, H. S.
700 1 $aANJOS, G. G. dos
700 1 $aOLIVEIRA, D. M.
773 $tIn: CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 4., 2007, Varginha, MG. Anais...Varginha: UFLA, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Algodão (CNPA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
