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2. | | SIMÕES, M.; BAHIA, D.; TORRES, K.; ZERLOTINI NETO, A.; ARTIGUENAVE, F.; FALCAO, P. R. K.; NESHICH, G.; OLIVEIRA, G. SNP identification in Schistosoma mansoni expressed genes. In: X-MEETING; INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu. [Proceedings...]. [S.l.]: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 131. X-meeting 2005. Presented Posters. Na publicação: Paula Kuser. Biblioteca(s): Embrapa Agricultura Digital. |
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3. | | SIMÕES, M.; BAHIA, D.; ZERLOTINI, A.; TORRES, K.; ARTIGUENAVE, F.; NESHICH, G.; KUSER, P.; OLIVEIRA, G. Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni. Molecular and Biochemical Parasitology, v. 154, p. 134-140, 2007. Biblioteca(s): Embrapa Agricultura Digital. |
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Registros recuperados : 3 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
28/08/2007 |
Data da última atualização: |
17/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
SIMÕES, M.; BAHIA, D.; ZERLOTINI, A.; TORRES, K.; ARTIGUENAVE, F.; NESHICH, G.; KUSER, P.; OLIVEIRA, G. |
Afiliação: |
MARIANA SIMÕES, Fiocruz; DIANA BAHIA, Fiocruz; ADHEMAR ZERLOTINI, Fiocruz; KLEIDER TORRES, Fiocruz; FRANÇOIS ARTIGUENAVE, Inra; GORAN NESHICH, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; GUILHERME OLIVEIRA, Fiocruz, Santa Casa de Belo Horizonte. |
Título: |
Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Molecular and Biochemical Parasitology, v. 154, p. 134-140, 2007. |
DOI: |
10.1016/j.molbiopara.2007.04.003 |
Idioma: |
Inglês |
Conteúdo: |
Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope |
Palavras-Chave: |
Bioinformática; Comparative modeling; Computational biology; Polimorfismo de nucleotídeo único. |
Thesagro: |
Schistosoma Mansoni. |
Thesaurus NAL: |
Bioinformatics; Cathepsin B; Models; Single nucleotide polymorphism. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02052naa a2200325 a 4500 001 1002699 005 2020-01-17 008 2007 bl uuuu u00u1 u #d 024 7 $a10.1016/j.molbiopara.2007.04.003$2DOI 100 1 $aSIMÕES, M. 245 $aSingle nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni.$h[electronic resource] 260 $c2007 520 $aSingle nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope 650 $aBioinformatics 650 $aCathepsin B 650 $aModels 650 $aSingle nucleotide polymorphism 650 $aSchistosoma Mansoni 653 $aBioinformática 653 $aComparative modeling 653 $aComputational biology 653 $aPolimorfismo de nucleotídeo único 700 1 $aBAHIA, D. 700 1 $aZERLOTINI, A. 700 1 $aTORRES, K. 700 1 $aARTIGUENAVE, F. 700 1 $aNESHICH, G. 700 1 $aKUSER, P. 700 1 $aOLIVEIRA, G. 773 $tMolecular and Biochemical Parasitology$gv. 154, p. 134-140, 2007.
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