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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/03/2022 |
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Data da última atualização: |
14/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUEDES, P. H. E.; PINNA, A. E.; MORAIS, R. de C. L.; SOUZA, G. N. de; JACOB, J. C. F.; CARVALHO, C. F. P. de M.; NOGUEIRA, L. A. G.; OLIVEIRA, C. S. |
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Afiliação: |
PEDRO HENRIQUE EVANGELISTA GUEDES, Universidade Federal Fluminense; ALINE EMERIM PINNA, Universidade Federal Fluminense; RITA DE CÁSSIA LIMA MORAIS, Universidade Federal Rural do Rio de Janeiro; GUILHERME NUNES DE SOUZA, CNPGL; JULIO CESAR FERRAZ JACOB, Universidade Federal Rural do Rio de Janeiro; CARLA FERNANDA PARANHOS DE MOURA CARVALHO, Universidade Federal Rural do Rio de Janeiro; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Doppler evaluation of recipient cows treated with progesterone and estradiol. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Animal Production Science, v. 62, n. 9, p. 836-843, 2022. |
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DOI: |
https://doi.org/10.1071/AN21298 |
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Idioma: |
Inglês |
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Conteúdo: |
Context: Due to the advances in the field of biotechnology of reproduction, success rates in embryo transfer (ET) programs have been growing continuously. Such rates are influenced by several factors, among which fertility and success in synchronising the estrus of the recipients stand out. Doppler ultrasound is emerging as a useful tool fot the assessment of fertility in recipients and for the early diagnosis of pregnancy. Aims. This study aimed to verify whether luteal vascularisation, uterine haemodynamics and corpus luteum (CL) volume, evaluated only on the day of fixed-time ET (FTET), are useful parameters for the selection of Girolando recipients synchronised with progesterone and estrogen. Methods. Girolando recipients received a hormonal protocol for FTET and were evaluated on the day of embryo transfer by B-mode and Doppler ultrasonography. The volume of the CL, the side of the CL, the estimation of luteal vascularisation and the Doppler indexes of the right and left uterine arteries were evaluated in real time. The ultrasound images were saved and used for later pixel counting and determination of the vascularised luteal area using specific software. Blastocysts(n = 92) produced in vitro (IVF) with Grade 1 morphology on Day 7 of development were transferred to all animals with corpus luteum. The pregnancy diagnosis was made 24 days after embryo transfer and the animals were separated into two groups, namely, pregnant and non-pregnant. Key results: Retrospective analyses of luteal vascularisation; haemodynamics of uterine arteries and corpus luteum volume did not differ (P > 0.05) between the groups. The means of the Doppler indexes of the uterine arteries were not affected (P > 0.05) by the presence of CL ipsilateral to the assessed vessel. Conclusions: It is concluded that the assessments of uterine haemodynamics, size and vascularisation of the CL, through Doppler ultrasonography on the day of ET, under the proposed conditions, were not useful to predict recipients with greater chances of establishing pregnancy. Implications: Although Doppler is a useful tool for real-time fertility assessment, its accuracy if affected by protocols that do not guarantee synchrony between ovulation times. This suggests that other fertility assessment methods be used under these field conditions. MenosContext: Due to the advances in the field of biotechnology of reproduction, success rates in embryo transfer (ET) programs have been growing continuously. Such rates are influenced by several factors, among which fertility and success in synchronising the estrus of the recipients stand out. Doppler ultrasound is emerging as a useful tool fot the assessment of fertility in recipients and for the early diagnosis of pregnancy. Aims. This study aimed to verify whether luteal vascularisation, uterine haemodynamics and corpus luteum (CL) volume, evaluated only on the day of fixed-time ET (FTET), are useful parameters for the selection of Girolando recipients synchronised with progesterone and estrogen. Methods. Girolando recipients received a hormonal protocol for FTET and were evaluated on the day of embryo transfer by B-mode and Doppler ultrasonography. The volume of the CL, the side of the CL, the estimation of luteal vascularisation and the Doppler indexes of the right and left uterine arteries were evaluated in real time. The ultrasound images were saved and used for later pixel counting and determination of the vascularised luteal area using specific software. Blastocysts(n = 92) produced in vitro (IVF) with Grade 1 morphology on Day 7 of development were transferred to all animals with corpus luteum. The pregnancy diagnosis was made 24 days after embryo transfer and the animals were separated into two groups, namely, pregnant and non-pregnant. Key results: Retrospective ana... Mostrar Tudo |
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Palavras-Chave: |
Fluxo sanguíneo; Hemodinâmica. |
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Thesagro: |
Corpo Lúteo; Fertilidade Animal; Gado Gir; Gado Holandês. |
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Thesaurus Nal: |
Girolando. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03217naa a2200301 a 4500
001 2141275
005 2022-06-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/AN21298$2DOI
100 1 $aGUEDES, P. H. E.
245 $aDoppler evaluation of recipient cows treated with progesterone and estradiol.$h[electronic resource]
260 $c2022
520 $aContext: Due to the advances in the field of biotechnology of reproduction, success rates in embryo transfer (ET) programs have been growing continuously. Such rates are influenced by several factors, among which fertility and success in synchronising the estrus of the recipients stand out. Doppler ultrasound is emerging as a useful tool fot the assessment of fertility in recipients and for the early diagnosis of pregnancy. Aims. This study aimed to verify whether luteal vascularisation, uterine haemodynamics and corpus luteum (CL) volume, evaluated only on the day of fixed-time ET (FTET), are useful parameters for the selection of Girolando recipients synchronised with progesterone and estrogen. Methods. Girolando recipients received a hormonal protocol for FTET and were evaluated on the day of embryo transfer by B-mode and Doppler ultrasonography. The volume of the CL, the side of the CL, the estimation of luteal vascularisation and the Doppler indexes of the right and left uterine arteries were evaluated in real time. The ultrasound images were saved and used for later pixel counting and determination of the vascularised luteal area using specific software. Blastocysts(n = 92) produced in vitro (IVF) with Grade 1 morphology on Day 7 of development were transferred to all animals with corpus luteum. The pregnancy diagnosis was made 24 days after embryo transfer and the animals were separated into two groups, namely, pregnant and non-pregnant. Key results: Retrospective analyses of luteal vascularisation; haemodynamics of uterine arteries and corpus luteum volume did not differ (P > 0.05) between the groups. The means of the Doppler indexes of the uterine arteries were not affected (P > 0.05) by the presence of CL ipsilateral to the assessed vessel. Conclusions: It is concluded that the assessments of uterine haemodynamics, size and vascularisation of the CL, through Doppler ultrasonography on the day of ET, under the proposed conditions, were not useful to predict recipients with greater chances of establishing pregnancy. Implications: Although Doppler is a useful tool for real-time fertility assessment, its accuracy if affected by protocols that do not guarantee synchrony between ovulation times. This suggests that other fertility assessment methods be used under these field conditions.
650 $aGirolando
650 $aCorpo Lúteo
650 $aFertilidade Animal
650 $aGado Gir
650 $aGado Holandês
653 $aFluxo sanguíneo
653 $aHemodinâmica
700 1 $aPINNA, A. E.
700 1 $aMORAIS, R. de C. L.
700 1 $aSOUZA, G. N. de
700 1 $aJACOB, J. C. F.
700 1 $aCARVALHO, C. F. P. de M.
700 1 $aNOGUEIRA, L. A. G.
700 1 $aOLIVEIRA, C. S.
773 $tAnimal Production Science$gv. 62, n. 9, p. 836-843, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
29/07/2004 |
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Data da última atualização: |
06/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, J. L. da S.; MENGE, J. A.; CASALE, W. L. |
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Afiliação: |
JEFFERSON LUIS DA SILVA COSTA, CNPAF; JOHN A. MENGE; WILLIAN L. CASALE. |
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Título: |
Biological control of Phytophthora root rot of avocado with microorganisms grown in organic mulches. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 31, n. 4, p. 239-246, Oct./Dec. 2000. |
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DOI: |
https://doi.org/10.1590/S1517-83822000000400002 |
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Idioma: |
Inglês |
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Conteúdo: |
Organic mulches colonized with microbial biocontrol agents, termed bioenhanced mulches, were tested for their ability to reduce Phytophthora root rot of avocado (Persea americana Mill.). Benomyl-resistant mutants of Gliocladium virens (KA 230-1) and Trichoderma harzianum (KA 159.2) isolated from suppressive soils and selected as efficient antagonists of P. cinnamomi were evaluated for their ability to colonize different mulches under controlled laboratory conditions. Sudangrass and a coarse yardwaste were found to be better substrates than a fine yardwaste, woodwaste or rice hulls for biocontrol agents propagules production. The most suitable conditions for colonization were an optimum temperature of 24°C, a moisture content of 20% for sudangrass and 30% for the coarse yardwaste, and a continuous light exposure during a 15-day incubation period. In the greenhouse, fresh sudangrass and a coarse yardwaste colonized with G. virens and used as a surface mulch proved to be the best combination for reducing the population of P. cinnamomi in 4-liter pots containing artificially-infested soil. Healthy avocado roots made up 31-37% of the roots in the G. virens-mulch combinations compared to 0% healthy in infested controls after two months. |
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Palavras-Chave: |
Avocado; Gliocladium virens; Phytophthora cinnamoni. |
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Thesagro: |
Trichoderma Harzianum. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/212442/1/CostaBJM.pdf
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Marc: |
LEADER 01942naa a2200205 a 4500
001 1212442
005 2022-04-06
008 2000 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1517-83822000000400002$2DOI
100 1 $aCOSTA, J. L. da S.
245 $aBiological control of Phytophthora root rot of avocado with microorganisms grown in organic mulches.$h[electronic resource]
260 $c2000
520 $aOrganic mulches colonized with microbial biocontrol agents, termed bioenhanced mulches, were tested for their ability to reduce Phytophthora root rot of avocado (Persea americana Mill.). Benomyl-resistant mutants of Gliocladium virens (KA 230-1) and Trichoderma harzianum (KA 159.2) isolated from suppressive soils and selected as efficient antagonists of P. cinnamomi were evaluated for their ability to colonize different mulches under controlled laboratory conditions. Sudangrass and a coarse yardwaste were found to be better substrates than a fine yardwaste, woodwaste or rice hulls for biocontrol agents propagules production. The most suitable conditions for colonization were an optimum temperature of 24°C, a moisture content of 20% for sudangrass and 30% for the coarse yardwaste, and a continuous light exposure during a 15-day incubation period. In the greenhouse, fresh sudangrass and a coarse yardwaste colonized with G. virens and used as a surface mulch proved to be the best combination for reducing the population of P. cinnamomi in 4-liter pots containing artificially-infested soil. Healthy avocado roots made up 31-37% of the roots in the G. virens-mulch combinations compared to 0% healthy in infested controls after two months.
650 $aTrichoderma Harzianum
653 $aAvocado
653 $aGliocladium virens
653 $aPhytophthora cinnamoni
700 1 $aMENGE, J. A.
700 1 $aCASALE, W. L.
773 $tBrazilian Journal of Microbiology$gv. 31, n. 4, p. 239-246, Oct./Dec. 2000.
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Embrapa Arroz e Feijão (CNPAF) |
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