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Registros recuperados : 6 | |
2. | | ROSA, J. DA; VIANA, A. J. C.; ROGGIA, I.; MORAES, J. S.; BUENO, A. de F.; MARIN, S. R. R.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Métodos de delivery de dsRNA para estudos de silenciamento gênico via RNAi em Euchistus heros (percevejo marrom) In: REUNIÃO DE PESQUISA DE SOJA, 39., 2024, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2024. p. 64-66. (Embrapa Soja. Eventos técnicos & científicos, 3). Organização da publicação: Regina Maria Villas Bôas de Campos Leite, Claudine Dinali Santos Seixas. RPS 2024. Biblioteca(s): Embrapa Soja. |
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3. | | COUTINHO, I. D.; FACCHINATO, W. M.; MERTZ-HENNING, L. M.; VIANA, A. J. C.; MARIN, S. R. R.; SANTAGNELI, S. H.; NEPOMUCENO, A. L.; COLNAGO, L. A. NMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors. ACS Omega, v. 9, 2024. 32651−32661 Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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4. | | ROSA, J. da; VIANA, A. J. C.; FERREIRA, F. R. A.; KOLTUN, A.; MERTZ-HENNING, L. M.; MARIN, S. R. R.; RECH FILHO, E. L.; NEPOMUCENO, A. L. Optimizing dsRNA engineering strategies and production in E. coli HT115 (DE3). Journal of Industrial Microbiology and Biotechnology, v. 51, kuae028, 2024. Na publicação: Elibio Leopoldo Rech. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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5. | | JOAQUIM, P. I. L.; MOLINARI, M. D. C.; MARIN, S. R. R.; BARBOSA, D. A.; VIANA, A. J. C.; RECH FILHO, E. L.; HENNING, F. A.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Nitrogen compounds transporters: candidates to increase the protein content in soybean seeds. Journal of Plant Interactions, v. 17, n. 1, p. 309-318, 2022. Na publicação: Elibio L. Rech. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | JOAQUIM, P. I. L.; MOLINARI, M. D. C.; MARIN, S. R. R.; BARBOSA, D. A.; VIANA, A. J. C.; RECH, E. L.; HENNING, F. A.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Nitrogen compounds transporters: candidates to increase the protein content in soybean seeds. Journal of Plant Interactions, v. 17, n. 1, p. 309-318, 2022. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 6 | |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação; Embrapa Soja. |
Data corrente: |
08/08/2024 |
Data da última atualização: |
28/08/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
COUTINHO, I. D.; FACCHINATO, W. M.; MERTZ-HENNING, L. M.; VIANA, A. J. C.; MARIN, S. R. R.; SANTAGNELI, S. H.; NEPOMUCENO, A. L.; COLNAGO, L. A. |
Afiliação: |
ISABEL DUARTE COUTINHO, INSTITUTO DE QUÍMICA DA UNESP-ARARAQUARA; WILLIAM MARCONDES FACCHINATTO, NSTITUTO DE QUÍMICA DE SÃO CARLOS, UNIVERSIDADE DE SÃO PAULO, IQSC/USP; LILIANE MARCIA MERTZ HENNING, CNPSO; AMÉRICO JOSÉ CARVALHO VIANA, UNIVERSIDADE ESTADUAL DE CAMPINAS (UNICAMP); SILVANA REGINA ROCKENBACH MARIN, CNPSO; SÃO PAULO STATE UNIVERSITY (UNESP); ALEXANDRE LIMA NEPOMUCENO, CNPSO; LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
NMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
ACS Omega, v. 9, 2024. |
Páginas: |
32651−32661 |
DOI: |
https://doi.org/10.1021/acsomega.4c01796 |
Idioma: |
Inglês |
Conteúdo: |
the insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism in soybean plants. Differential responses were observed among roots and leaves as well as drought-tolerant and drought-sensitive cultivars, highlighting the complex interplay between overexpressed transcription factors and drought stress in soybean plants. Menosthe insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism i... Mostrar Tudo |
Palavras-Chave: |
Cultivar BR16. |
Thesagro: |
Espectrometria; Física; Soja. |
Thesaurus NAL: |
Nuclear magnetic resonance spectroscopy; Soybeans. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1166368/1/P-NMR-Fingerprinting-of-Conventional-and-Genetically-Modified.pdf
|
Marc: |
LEADER 02648naa a2200301 a 4500 001 2166368 005 2024-08-28 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1021/acsomega.4c01796$2DOI 100 1 $aCOUTINHO, I. D. 245 $aNMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors.$h[electronic resource] 260 $c2024 300 $a32651−32661 520 $athe insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism in soybean plants. Differential responses were observed among roots and leaves as well as drought-tolerant and drought-sensitive cultivars, highlighting the complex interplay between overexpressed transcription factors and drought stress in soybean plants. 650 $aNuclear magnetic resonance spectroscopy 650 $aSoybeans 650 $aEspectrometria 650 $aFísica 650 $aSoja 653 $aCultivar BR16 700 1 $aFACCHINATO, W. M. 700 1 $aMERTZ-HENNING, L. M. 700 1 $aVIANA, A. J. C. 700 1 $aMARIN, S. R. R. 700 1 $aSANTAGNELI, S. H. 700 1 $aNEPOMUCENO, A. L. 700 1 $aCOLNAGO, L. A. 773 $tACS Omega$gv. 9, 2024.
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