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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Gado de Corte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
28/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
SIHLER, W.; SANCHES, M. M.; FAZOLIN, M.; ESTRELA, J. L. V.; SOUZA, M. L. de. |
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Afiliação: |
WILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen. |
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Título: |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Technology in Agronomy, v. 4, e001, 2024. |
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DOI: |
https://doi.org/10.48130/tia-0023-0019 |
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Idioma: |
Inglês |
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Conteúdo: |
The hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations. MenosThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparis... Mostrar Tudo |
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Palavras-Chave: |
Acre; Amazonia Occidental; Amazônia Ocidental; Árbol de goma; Caterpillar; Comunidades rurales; Control biológico; Cruzeiro do Sul (AC); Cultivo a pequeña escala; Hornworm; Plagas de plantas; Rubber tree; Western Amazon; x; Yuca. |
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Thesagro: |
Baculovirus; Controle Biológico; Erinnyis Ello; Hevea Brasiliensis; Lagarta; Mandioca; Manihot Esculenta; Pequeno Produtor; Praga de Planta; Propriedade Rural; Seringueira. |
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Thesaurus NAL: |
Baculoviridae; Biological control; Cassava; DsDNA viruses; Insect larvae; Lepidoptera; Pest management; Plant pests; Rural communities; Small-scale farming. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1162096/1/tia-0023-0019.pdf
https://ainfo.cnptia.embrapa.br
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Marc: |
LEADER 03389naa a2200613 a 4500
001 2162337
005 2024-02-28
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.48130/tia-0023-0019$2DOI
100 1 $aSIHLER, W.
245 $aErinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.$h[electronic resource]
260 $c2024
520 $aThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations.
650 $aBaculoviridae
650 $aBiological control
650 $aCassava
650 $aDsDNA viruses
650 $aInsect larvae
650 $aLepidoptera
650 $aPest management
650 $aPlant pests
650 $aRural communities
650 $aSmall-scale farming
650 $aBaculovirus
650 $aControle Biológico
650 $aErinnyis Ello
650 $aHevea Brasiliensis
650 $aLagarta
650 $aMandioca
650 $aManihot Esculenta
650 $aPequeno Produtor
650 $aPraga de Planta
650 $aPropriedade Rural
650 $aSeringueira
653 $aAcre
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aÁrbol de goma
653 $aCaterpillar
653 $aComunidades rurales
653 $aControl biológico
653 $aCruzeiro do Sul (AC)
653 $aCultivo a pequeña escala
653 $aHornworm
653 $aPlagas de plantas
653 $aRubber tree
653 $aWestern Amazon
653 $ax
653 $aYuca
700 1 $aSANCHES, M. M.
700 1 $aFAZOLIN, M.
700 1 $aESTRELA, J. L. V.
700 1 $aSOUZA, M. L. de
773 $tTechnology in Agronomy$gv. 4, e001, 2024.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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