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| Registros recuperados : 4 | |
| 1. |  | SILVA, V. S.; LOPES, K. G. S.; BERTANI, G. R.; OLIVEIRA FILHO, E. F. de; TREVISOL, I. M.; KRAMER, B.; COLDEBELLA, A.; GIL, L. H. V. G. Seroprevalence of hepatitis e virus (HEV) in domestic non-commercial pigs reared in small-scale farms and wild boar in south of Brazil. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF BIOLOGICAL, CHEMICAL AND PHYSICAL HAZARDS IN PIG AND PORK, 12., 2017, Foz do Iguaçu. Proceedings Book. Concórdia: Embrapa Suínos e Aves, 2017. p. 72-75. SafePork 2017.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SILVA, T. R.; SOARES, P. C.; DANTAS, A. F. M.; MARQUES. A. V. S.; OLIVEIRA FILHO, E. F.; AGUIAR, G. M. N.; MARQUES, A. L. A.; RIET-CORREA, F. Serum and liver copper, iron, molybdenum and zinc concentration in goats and sheep in the state of Paraíba, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1313-1316, julho 2018 Título em português: Concentrações sérica e hepática de cobre, ferro, molibdênio e zinco em ovinos e caprinos no estado da Paraíba.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | OLIVEIRA FILHO, E. F. de; LOPES, K. G. S.; CUNHA, D. S.; SILVA, V. S.; BARBOSA, C. N.; BRANDESPIM, D. F.; PINHEIRO JUNIOR, J. W.; BERTANI, G. R.; GIL, L. H. V. G. Risk analysis and occurrence of Hepatitis E Virus (HEV) in domestic swine in northeast Brazil. Food and Environmental Virology, v. 9, p. 256-259, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | MENEZES, V. G.; MONTE, A. P. O.; BARBERINO, R. de S.; GOUVEIA, B. B.; LINS, T. L. B.; MACEDO, T. J. S. e; OLIVEIRA JÚNIOR, J. L. de; ARAÚJO, C. de A.; CAMPOS, F. S.; OLIVEIRA FILHO, E. F. de; BATISTA, A. M.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. de; WISCHRA, A. Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. Animal Reproduction Science, v. 255, 107278, Jun. 2023.| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/09/2023 |
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Data da última atualização: |
19/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MENEZES, V. G.; MONTE, A. P. O.; BARBERINO, R. de S.; GOUVEIA, B. B.; LINS, T. L. B.; MACEDO, T. J. S. e; OLIVEIRA JÚNIOR, J. L. de; ARAÚJO, C. de A.; CAMPOS, F. S.; OLIVEIRA FILHO, E. F. de; BATISTA, A. M.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. de; WISCHRA, A. |
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Afiliação: |
VANÚZIA GONÇALVES MENEZES, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; BRUNA BORTOLONI GOUVEIA, UNIVASF; THAE LANE BARBOSA GAMA LINS, UNIVASF; TAÍS JOBARD SILVA E MACEDO, UNIVASF; JOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASF; CLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PE; FLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MA; EMANUEL FELIPE DE OLIVEIRA FILHO, UFRPE; ANDRÉ MARIANO BATISTA, UFRPE; MARIO ADRIANO ÁVILA QUEIROZ, UNIVASF; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; MARIA HELENA TAVARES DE MATOS, UNIVASF; AUREA WISCHRA, UFRPE. |
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Título: |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 255, 107278, Jun. 2023. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2023.107278 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes MenosThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicle... Mostrar Tudo |
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Palavras-Chave: |
Foliculogênese; Folículos primordiais; Hormônios sexuais; Oócito. |
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Thesagro: |
Água Potável; Ovelha. |
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Thesaurus NAL: |
Drinking water; Oocytes; Sex hormones; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156781/1/Effect-of-reduced-water-intake-on-ovarian-reserve-leptin-Ovelha-Foliculo-2023.pdf
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Marc: |
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001 2156781
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024 7 $ahttps://doi.org/10.1016/j.anireprosci.2023.107278$2DOI
100 1 $aMENEZES, V. G.
245 $aEffect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.$h[electronic resource]
260 $c2023
520 $aThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
650 $aDrinking water
650 $aOocytes
650 $aSex hormones
650 $aSheep
650 $aÁgua Potável
650 $aOvelha
653 $aFoliculogênese
653 $aFolículos primordiais
653 $aHormônios sexuais
653 $aOócito
700 1 $aMONTE, A. P. O.
700 1 $aBARBERINO, R. de S.
700 1 $aGOUVEIA, B. B.
700 1 $aLINS, T. L. B.
700 1 $aMACEDO, T. J. S. e
700 1 $aOLIVEIRA JÚNIOR, J. L. de
700 1 $aARAÚJO, C. de A.
700 1 $aCAMPOS, F. S.
700 1 $aOLIVEIRA FILHO, E. F. de
700 1 $aBATISTA, A. M.
700 1 $aQUEIROZ, M. A. A.
700 1 $aARAUJO, G. G. L. de
700 1 $aMATOS, M. H. T. de
700 1 $aWISCHRA, A.
773 $tAnimal Reproduction Science$gv. 255, 107278, Jun. 2023.
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Embrapa Semiárido (CPATSA) |
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