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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166571/1/Identification-of-new-Corynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
28/11/2021 |
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Data da última atualização: |
28/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
COSTA, D. P. da; CASTILHO, C. de L.; SANTOS, U. R. A. dos; GEMAQUE, T. C.; DAMASCENO, L. F.; LIMA, J. de F.; MIRANDA FILHO, K. C. |
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Afiliação: |
DANIEL PEREIRA DA COSTA, UEAP; CLAUDIANA DE LIMA CASTILHO, UFRA; UCLÉDIA ROBERTA ALBERTO DOS SANTOS, UFSC; TAINÁRA CUNHA GEMAQUE, UFMG; LEANDRO FERNANDES DAMASCENO, CPAF-AP; JO DE FARIAS LIMA, CPAF-AP; KLEBER CAMPOS MIRANDA FILHO, UFMG. |
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Título: |
Effect of dietary carotenoids on reproducers of Amazon River prawn Macrobrachium amazonicum. Part 1: metabolism,morphometric / zootechnical indexes, body composition and gametes. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Agricultural Studies, v. 9, n. 2, p. 32-56, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
The inclusion of natural extracts and solution of synthetic astaxan thin in the Macrobrachium amazonicum diet w ere tested to verify their effect on performance, body indexes and total accumulation of astaxanthin in the reproductive tissues and gametes of this crustacean in comparison with newly captured wild animals. The e xperi ment was randomized in blocks (five treatments with three replicates). Four groups were submitted, during 20 days, to diets in recirculation tanks: control diet (CONT); diet containing natural extract of “buriti” (CAR); diet with inclusion of natural “uruc um” extract (BIXN), diet with syntheti c astaxanthin (ASTX) and NATURAL group (not fed with ration). 180 prawns were used, with 60 males (6.08±1.96 g) and 120 females (4.55±1.03 g) distributed in five groups containing four males and eight females each . The re were no significant differences in performance and body indexes. The number of released spermatozoids, live spermatozoids, body and egg pigmentation was higher in BIXN and ASTX treatments. The ASTX treatment was superior to the NATURAL group in the body pigmentation of females and eggs and release of spermatophores by males. These results demonstrate that the use of natural and artificial carotenoid pigments in the diet are beneficial for reproduction of M. amazonicum. |
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Palavras-Chave: |
Electroejaculation; Prawn culture. |
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Thesagro: |
Buriti; Urucum. |
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Thesaurus NAL: |
Astaxanthin; Reproductive performance. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228190/1/CPAF-AP-2021-Effect-of-dietary-carotenoids-part-1.pdf
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Marc: |
LEADER 02205naa a2200265 a 4500
001 2136703
005 2021-11-28
008 2021 bl uuuu u00u1 u #d
100 1 $aCOSTA, D. P. da
245 $aEffect of dietary carotenoids on reproducers of Amazon River prawn Macrobrachium amazonicum. Part 1$bmetabolism,morphometric / zootechnical indexes, body composition and gametes.$h[electronic resource]
260 $c2021
520 $aThe inclusion of natural extracts and solution of synthetic astaxan thin in the Macrobrachium amazonicum diet w ere tested to verify their effect on performance, body indexes and total accumulation of astaxanthin in the reproductive tissues and gametes of this crustacean in comparison with newly captured wild animals. The e xperi ment was randomized in blocks (five treatments with three replicates). Four groups were submitted, during 20 days, to diets in recirculation tanks: control diet (CONT); diet containing natural extract of “buriti” (CAR); diet with inclusion of natural “uruc um” extract (BIXN), diet with syntheti c astaxanthin (ASTX) and NATURAL group (not fed with ration). 180 prawns were used, with 60 males (6.08±1.96 g) and 120 females (4.55±1.03 g) distributed in five groups containing four males and eight females each . The re were no significant differences in performance and body indexes. The number of released spermatozoids, live spermatozoids, body and egg pigmentation was higher in BIXN and ASTX treatments. The ASTX treatment was superior to the NATURAL group in the body pigmentation of females and eggs and release of spermatophores by males. These results demonstrate that the use of natural and artificial carotenoid pigments in the diet are beneficial for reproduction of M. amazonicum.
650 $aAstaxanthin
650 $aReproductive performance
650 $aBuriti
650 $aUrucum
653 $aElectroejaculation
653 $aPrawn culture
700 1 $aCASTILHO, C. de L.
700 1 $aSANTOS, U. R. A. dos
700 1 $aGEMAQUE, T. C.
700 1 $aDAMASCENO, L. F.
700 1 $aLIMA, J. de F.
700 1 $aMIRANDA FILHO, K. C.
773 $tJournal of Agricultural Studies$gv. 9, n. 2, p. 32-56, 2021.
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Embrapa Amapá (CPAF-AP) |
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