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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
12/12/2016 |
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Data da última atualização: |
12/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, J. D.; CANTAO, M. E.; CESAR, D. E.; NICOLÁS, M. F.; DINIZ, C. G.; SILVA, V. L.; VASCONCELOS, A. T. R. de; COELHO, C. M. |
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Afiliação: |
JULLIANE DUTRA MEDEIROS, UFMG/ICB; MAURICIO EGIDIO CANTAO, CNPSA; DIONÉIA EVANGELISTA CESAR, UFJF; MARISA FABIANA NICOLÁS, Laboratório Nacional de Computação Científica; CLÁUDIO GALUPPO DINIZ, UFJF; VÂNIA LÚCIA SILVA, UFJF; ANA TEREZA RIBEIRO DE VASCONCELOS, Laboratório Nacional de Computação Científica; CÍNTIA MARQUES COELHO, UFMG/ICB. |
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Título: |
Comparative metagenome of a stream impacted by the urbanization phenomenon. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, SP, v. 47, n. 4, p. 835-845, 2016. |
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DOI: |
http://dx.doi.org/10.1016/j.bjm.2016.06.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rivers and streams are important reservoirs of freshwater for human consumption. Theseecosystems are threatened by increasing urbanization, because raw sewage dischargedinto them alters their nutrient content and may affect the composition of their micro-bial community. In the present study, we investigate the taxonomic and functional profileof the microbial community in an urban lotic environment. Samples of running waterwere collected at two points in the São Pedro stream: an upstream preserved and non-urbanized area, and a polluted urbanized area with discharged sewage. The metagenomicDNA was sequenced by pyrosequencing. Differences were observed in the communitycomposition at the two sites. The non-urbanized area was overrepresented by genera ofubiquitous microbes that act in the maintenance of environments. In contrast, the urban-ized metagenome was rich in genera pathogenic to humans. The functional profile indicatedthat the microbes act on the metabolism of methane, nitrogen and sulfur, especially in theurbanized area. It was also found that virulence/defense (antibiotic resistance and metalresistance) and stress response-related genes were disseminated in the urbanized envi-ronment. The structure of the microbial community was altered by uncontrolled anthropicinterference, highlighting the selective pressure imposed by high loads of urban sewagedischarged into freshwater environments. |
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Thesagro: |
Melhoramento genético animal. |
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Thesaurus Nal: |
Animal breeding. |
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Categoria do assunto: |
-- |
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Marc: |
null
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
10/08/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, M. R.; FERREIRA, F. C.; MARANHÃO, A. G.; LANZARINI, N. M.; CASTRO, K. N. de C.; MIAGOSTOVICH, M. P. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL; FERNANDO CÉSAR FERREIRA, Instituto Oswaldo Cruz; ADRIANA GONÇALVES MARANHÃO, Instituto Oswaldo Cruz; NATÁLIA MARIA LANZARINI, Instituto Oswaldo Cruz; KARINA NEOOB DE CARVALHO CASTRO, CPATC; MARIZE PEREIRA MIAGOSTOVICH, Instituto Oswaldo Cruz. |
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Título: |
Assessment of viral contamination of five Brazilian artisanal cheese produced from raw milk: a randomized survey. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Food and Environmental Virology, v. 13, n. 4, p. 528-534, 2021. |
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DOI: |
https://doi.org/10.1007/s12560-021-09491-z |
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Idioma: |
Inglês |
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Conteúdo: |
Enteric viruses have been described as important contaminants in fresh and ready-to-eat foods such as sandwiches, deli meat and dairy products. This is a cross-sectional randomized survey to estimate the prevalence of norovirus and human adenovirus (HAdV) from 100 Brazilian artisanal raw milk cheese samples (Minas and Coalho) obtained from different agroindustries in four producing regions in the states of Minas Gerais and one in Piauí, respectively. From October 2017 to April 2018, norovirus genogroups I and II and HAdV were investigated in these cheese samples by RT-qPCR and qPCR, respectively. Viruses were detected in 43 samples, being 26 norovirus GI strains, 14 HAdV, and 3 both viruses. Norovirus GII strains were not detected. Viral concentrations ranged from 6.17 × 104 to 1.44 × 107 genome copies/L?1 and murine norovirus 1 used as internal process control showed 100% success rate of recovery with efficiency of 10%. There was a trend towards a higher positivity rate for both viruses in the rainy season, and HAdV were more commonly found among samples with higher fecal coliform counts. This study is a first step in assessing the risk that this contamination may pose to the consumer of raw products as well as emphasizing the need for good manufacturing practices, quality control systems in the dairy industry and markets. As a randomized survey, we established baseline figures for viruses? prevalence in five types of ready-to-eat raw milk artisanal Brazilian cheese, to allow any monitoring trends, setting control targets and future local risk analyses studies. MenosEnteric viruses have been described as important contaminants in fresh and ready-to-eat foods such as sandwiches, deli meat and dairy products. This is a cross-sectional randomized survey to estimate the prevalence of norovirus and human adenovirus (HAdV) from 100 Brazilian artisanal raw milk cheese samples (Minas and Coalho) obtained from different agroindustries in four producing regions in the states of Minas Gerais and one in Piauí, respectively. From October 2017 to April 2018, norovirus genogroups I and II and HAdV were investigated in these cheese samples by RT-qPCR and qPCR, respectively. Viruses were detected in 43 samples, being 26 norovirus GI strains, 14 HAdV, and 3 both viruses. Norovirus GII strains were not detected. Viral concentrations ranged from 6.17 × 104 to 1.44 × 107 genome copies/L?1 and murine norovirus 1 used as internal process control showed 100% success rate of recovery with efficiency of 10%. There was a trend towards a higher positivity rate for both viruses in the rainy season, and HAdV were more commonly found among samples with higher fecal coliform counts. This study is a first step in assessing the risk that this contamination may pose to the consumer of raw products as well as emphasizing the need for good manufacturing practices, quality control systems in the dairy industry and markets. As a randomized survey, we established baseline figures for viruses? prevalence in five types of ready-to-eat raw milk artisanal Brazilian cheese, to all... Mostrar Tudo |
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Palavras-Chave: |
HAdV; Leite cru. |
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Thesagro: |
Adenovírus; Análise de Risco; Contaminação; Laticínio; Leite; Queijo; Vírus. |
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Thesaurus NAL: |
Cheeses; Norovirus; Raw milk. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
null
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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