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| Registros recuperados : 5 | |
| 3. |  | SILVA, B. D. M.; MELO, E. O.; SARTORI, R.; MAXIMO, D. M.; MOURA, P.; FIORILLO, K. S.; SILVA, T. A. S. N.; NEVES, J. P. Influência de uma nova mutação do gene GDF-9 (growth and differentiation factor 9) na taxa de ovulação em ovelhas da raça Santa Inês. Acta Scientiae Veterinariae, Porto Alegre, v. 35, supl. 3, p. S974, 2007. Edição dos resumos do XXI Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador, BA, ago. 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | FIDELIS, A. A.; DRESCH, R. A.; SIQUEIRA FILHO, E.; MÁXIMO, D. M.; CARVALHO, J. O.; LIM, A. I. P. G.; BLUME, H.; RUMPF, R.; MONDADORI, R. G. Produção In vitro de embriões bubalinos em um sistema comercialmente utilizado em bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S499, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | REBELO, R.; MÁXIMO, D.; CHAM, K.; DIAS, C.; VIANA-SILVA, F.; BORGES, L.; TEIXEIRA, I.; SOUZA, R.; MARCONDES, C.; BELCHIOR, C.; PIRES, C. S. S.; SCORZA JUNIOR, R.; WAICHMAN, A.; OLIVEIRA, R.; MALASPINA, O.; NOCELLI, R.; TORNISIELO, A.; CIONE, A.; SHIWA, A.; MURAKAMI, L.; PATINO, X.; FERRAZ, A.; GUIMARÃES, G.; TONELLI, C. Research needs to improve ecological risk assessment in Brazil. In: SETAC Latin America, 12., BiennalMeeting, 2017, Santos, São Paulo. Abstract Book... Santos, SP: Society of Environmental toxicology and chemistry (SETAC), 2017. resumo 107. 41| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
07/08/2020 |
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Data da última atualização: |
27/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PINHEIRO, D. H.; MOREIRA, R. O.; LEITE, N. A.; REDOAN, A. C.; XAVIER, A. da S.; BARROS, B. de A.; CARNEIRO, N. P. |
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Afiliação: |
Daniele Heloisa Pinheiro; Raquel Oliveira Moreira, UNESP; Natalia Alves Leite, Universidade Federal do Rio Grande do Sul; Ana Carolina Redoan; Andre da Silva Xavier, Universidade Federal do Espírito Santo; BEATRIZ DE ALMEIDA BARROS, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 47, p. 4989-5000, 2020. |
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DOI: |
10.1007/s11033-020-05550-z |
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Idioma: |
Inglês |
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Conteúdo: |
The relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies. MenosThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the develop... Mostrar Tudo |
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Thesagro: |
Gene; Inseto; Percevejo; Seleção Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02446naa a2200253 a 4500
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024 7 $a10.1007/s11033-020-05550-z$2DOI
100 1 $aPINHEIRO, D. H.
245 $aSuitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera$bPentatomidae).$h[electronic resource]
260 $c2020
520 $aThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies.
650 $aGene
650 $aInseto
650 $aPercevejo
650 $aSeleção Genética
700 1 $aMOREIRA, R. O.
700 1 $aLEITE, N. A.
700 1 $aREDOAN, A. C.
700 1 $aXAVIER, A. da S.
700 1 $aBARROS, B. de A.
700 1 $aCARNEIRO, N. P.
773 $tMolecular Biology Reports$gv. 47, p. 4989-5000, 2020.
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