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Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br.
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Biblioteca(s):  Embrapa Instrumentação.
Data corrente:  30/06/2025
Data da última atualização:  30/06/2025
Tipo da produção científica:  Artigo em Periódico Indexado
Autoria:  LYSAK, D. H.; MICHAL, C. A.; MARABLE, K.; GRISI, M.; FARSI, R.; COSTA, P. M.; PELLIZZARI, J.; MOXLEY-PAQUETE, V.; DOWNEY, K.; RONDA, K.; MEULEN, O. V.; WOLF, W. W.; STEINER, K.; KOCK, F. V. C.; MORAES, T. B.; COLNAGO, L. A.; SCHUDERER, F.; WIRTH, T.; SIMPSON, M.; ANDERS, J.
Afiliação:  UNIVERSITY OF TORONTO SCARBOROUGH; DEPARTMENT OF PHYSICS AND ASTRONOMY, UNIVERSITY OF BRITISH COLUMBIA; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSIDADE DE SÃO PAULO; LUIZ ALBERTO COLNAGO, CNPDIA; UNIVERSITY OF STUTTGART; UNIVERSITY OF STUTTGART; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSITY OF STUTTGART.
Título:  Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples.
Ano de publicação:  2025
Fonte/Imprenta:  Analitical Chemistry, v. 97, 2025.
Páginas:  11643−11652
DOI:  https://doi.org/10.1021/acs.analchem.5c00955
Idioma:  Inglês
Conteúdo:  ABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs c... Mostrar Tudo
Palavras-Chave:  D magna eggs.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Instrumentação (CNPDIA)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CNPDIA18724 - 1UPCAP - DDPROCI. 25/612025/66
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Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br.

Registro Completo

Biblioteca(s):  Embrapa Semiárido.
Data corrente:  08/06/2020
Data da última atualização:  12/06/2020
Tipo da produção científica:  Artigo em Periódico Indexado
Circulação/Nível:  A - 1
Autoria:  CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M.
Afiliação:  Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar.
Título:  Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.
Ano de publicação:  2020
Fonte/Imprenta:  Food Research International, v. 131, may 2020.
DOI:  https://doi.org/10.1016/j.foodres.2019.108532
Idioma:  Inglês
Conteúdo:  Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo
Palavras-Chave:  Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação.
Thesagro:  Pectina; Subproduto; Uva.
Thesaurus NAL:  Grapes; Polysaccharides.
Categoria do assunto:  F Plantas e Produtos de Origem Vegetal
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Semiárido (CPATSA)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
CPATSA59142 - 1UPCAP - DD
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