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| 8. |  | OLIVEIRA, S. da C. de; GIRARDI, E. A.; FERREIRA JUNIOR, A. J.; BALARDIN, D. F. da R.; FREIRE, J. B. da R.; RIBEIRO, N. V. da S.; FRANCO, R. de J. Efeito de propágulos e fitorregulador na estaquia de amoreira (Morus nigra L.). In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
28/10/2019 |
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Data da última atualização: |
15/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RIBEIRO, N. V. da S.; VIDAL, M. S.; BARRIOS, S. C. L.; BALDANI, J. I. |
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Afiliação: |
NATALIA VIRGINIA DA SILVA RIBEIRO, UFRRJ; MARCIA SOARES VIDAL, CNPAB; SANZIO CARVALHO LIMA BARRIOS, CNPGC; JOSE IVO BALDANI, CNPAB. |
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Título: |
Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Plant and Soil, First Online: 27 August 2019 |
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ISSN: |
1573-5036 |
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DOI: |
https://doi.org/10.1007/s11104-019-04263-y |
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Idioma: |
Inglês |
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Notas: |
Publicado na Plant and Soil, v. 451, p. 187–205, 2020. |
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Conteúdo: |
Background and aims Diazotrophic bacteria, including those of the genus Azospirillum and Nitrospirillum, colonize Brachiaria genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from Brachiaria genotypes. Methods Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20 Brachiaria genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and nifH sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose. Results The diazotrophic population ranged from 102 to 108 g?1 rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera Azospirillum and Nitrospirillum, and revealed the presence of 14 other diazotrophic genera. The genus Nitrospirillum was detected colonizing all niches of most Brachiaria species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation. Conclusions The results showed a high diversity of diazotrophic bacterial species colonizing 20 Brachiaria genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizer MenosBackground and aims Diazotrophic bacteria, including those of the genus Azospirillum and Nitrospirillum, colonize Brachiaria genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from Brachiaria genotypes. Methods Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20 Brachiaria genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and nifH sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose. Results The diazotrophic population ranged from 102 to 108 g?1 rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera Azospirillum and Nitrospirillum, and revealed the presence of 14 other diazotrophic genera. The genus Nitrospirillum was detected colonizing all niches of most Brachiaria species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation. Conclusions The results showed a high diversity of diazotrophic bacterial species colonizing 20 Brachiaria genotypes and revealed the presence of bacteria with varia... Mostrar Tudo |
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Palavras-Chave: |
Diazotrophic bacteria; Gene Sequencing; Phylogenetic positioning. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02412naa a2200229 a 4500
001 2113534
005 2020-09-15
008 2019 bl uuuu u00u1 u #d
022 $a1573-5036
024 7 $ahttps://doi.org/10.1007/s11104-019-04263-y$2DOI
100 1 $aRIBEIRO, N. V. da S.
245 $aGenetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp.$h[electronic resource]
260 $c2019
500 $aPublicado na Plant and Soil, v. 451, p. 187–205, 2020.
520 $aBackground and aims Diazotrophic bacteria, including those of the genus Azospirillum and Nitrospirillum, colonize Brachiaria genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from Brachiaria genotypes. Methods Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20 Brachiaria genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and nifH sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose. Results The diazotrophic population ranged from 102 to 108 g?1 rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera Azospirillum and Nitrospirillum, and revealed the presence of 14 other diazotrophic genera. The genus Nitrospirillum was detected colonizing all niches of most Brachiaria species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation. Conclusions The results showed a high diversity of diazotrophic bacterial species colonizing 20 Brachiaria genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizer
653 $aDiazotrophic bacteria
653 $aGene Sequencing
653 $aPhylogenetic positioning
700 1 $aVIDAL, M. S.
700 1 $aBARRIOS, S. C. L.
700 1 $aBALDANI, J. I.
773 $tPlant and Soil, First Online: 27 August 2019
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