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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
12/12/2013 |
Data da última atualização: |
14/02/2014 |
Autoria: |
FIRMINO, A. C.; TOZZE JUNIOR, H. J.; SOUZA, I. C. G. de; FURTADO, E. L. |
Título: |
Resistência de genótipos de Eucalipto a Ceratocystis spp. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Scientia Forestalis, Piracicaba, v. 41, n. 98, p. 165-173, jun. 2013. |
Idioma: |
Português |
Palavras-Chave: |
Eucalipto sp; Eucalyptus sp; Murcha de eucalipto; Resistance; Wilt in eucalypts. |
Thesagro: |
Resistência. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00623naa a2200217 a 4500 001 1973696 005 2014-02-14 008 2013 bl uuuu u00u1 u #d 100 1 $aFIRMINO, A. C. 245 $aResistência de genótipos de Eucalipto a Ceratocystis spp. 260 $c2013 650 $aResistência 653 $aEucalipto sp 653 $aEucalyptus sp 653 $aMurcha de eucalipto 653 $aResistance 653 $aWilt in eucalypts 700 1 $aTOZZE JUNIOR, H. J. 700 1 $aSOUZA, I. C. G. de 700 1 $aFURTADO, E. L. 773 $tScientia Forestalis, Piracicaba$gv. 41, n. 98, p. 165-173, jun. 2013.
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Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
11/07/2018 |
Data da última atualização: |
11/07/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAMALHO, S. R.; BEZERRA, C. da S.; LIMA, L. S.; MARIA NETO, S.; OLIVEIRA, C. F. R. de; VERBISCK, N. V.; MACEDO, M. L. R. |
Afiliação: |
Suellen Rodrigues Ramalho; Cezar da Silva Bezerra; Letícia Souza Lima; Simone Maria Neto; Caio Fernando Ramalho de Oliveira; NEWTON VALERIO VERBISCK, CNPGC; Maria Lígia Rodrigues Macedo. |
Título: |
Novel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Journal Agricultural Food Chemistry. v. 66, n. 6, p. 1349-1358, fev. 2018. |
Idioma: |
Português |
Conteúdo: |
A novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control. |
Palavras-Chave: |
Kunitz-type inhibitor; Pest insect. |
Thesaurus NAL: |
Fabaceae; Trypsin inhibitors. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01706naa a2200241 a 4500 001 2093186 005 2018-07-11 008 2018 bl uuuu u00u1 u #d 100 1 $aRAMALHO, S. R. 245 $aNovel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae.$h[electronic resource] 260 $c2018 520 $aA novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control. 650 $aFabaceae 650 $aTrypsin inhibitors 653 $aKunitz-type inhibitor 653 $aPest insect 700 1 $aBEZERRA, C. da S. 700 1 $aLIMA, L. S. 700 1 $aMARIA NETO, S. 700 1 $aOLIVEIRA, C. F. R. de 700 1 $aVERBISCK, N. V. 700 1 $aMACEDO, M. L. R. 773 $tJournal Agricultural Food Chemistry.$gv. 66, n. 6, p. 1349-1358, fev. 2018.
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