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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
06/06/2018 |
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Data da última atualização: |
06/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MARTINS, D de S.; WATTHIER, M.; SCHWENGBER, J. E.; SILVA, D. R da; SCHUBERT, R. N.; PEIL, R. M. N. |
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Afiliação: |
DENISE DE SOUZA MARTINS; MARISTELA WATTHIER, UFV; JOSE ERNANI SCHWENGBER, CPACT; DIEGO ROSA DA SILVA; RYAN NOREMBERG SCHUBERT; ROBERTA MARINS NOGUEIRA PEIL, UFPEL. |
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Título: |
Planting density and number of stems for ecological crop determinate growth tomato. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
African Journal of Agricultural Research, v. 13, n. 12, p. 544-550, Mar. 2018. |
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ISSN: |
1991-637X |
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Idioma: |
Inglês |
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Conteúdo: |
Tomato growers adopting an ecologically based system have opted for determinate growth varieties due to their ease of staking and sprouting, and due to the fact that they have a shorter cycle, which reduces phytosanitary problems that usually occur towards the end of the growing season. This study aimed to evaluate yield components and fruit quality of ‘Floradade’ determinate growth tomato in an ecologically based production system with regard to plant density and number of stems per plant in two growing seasons, spring-summer (SS) and summer-fall (SF). Two experiments were conducted in Pelotas in the years 2010/2011 under open field conditions. Plants were trained with two or four stems and four plant densities were evaluated: 2.0; 2.5; 3.0 and 3.6 plants m-2in SS, and 1.5; 2.0; 2.5 and 3.0 plants m-2 in SF. The fruit number, fruit average weight, fruit yield, ascorbic acid content, total soluble solids and fruit pH were evaluated. The average fruit yield obtained in SF was on average 80% lower than that in SS. The increase of plant density and number of stems per plant led to an increase in fruit number and fruit yield in the two crop seasons. There was an increase in total soluble solids and reduction in the ascorbic acid content of the fruit. Two stemsper plant and plant density of 3.0 plants m-2 are recommended for the ‘Floradade’ tomato crop under an ecological production system and SS crop conditions. |
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Thesagro: |
Tomate. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178194/1/Artigo-African-Journal.pdf
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Marc: |
LEADER 02056naa a2200205 a 4500
001 2092257
005 2018-06-06
008 2018 bl uuuu u00u1 u #d
022 $a1991-637X
100 1 $aMARTINS, D de S.
245 $aPlanting density and number of stems for ecological crop determinate growth tomato.$h[electronic resource]
260 $c2018
520 $aTomato growers adopting an ecologically based system have opted for determinate growth varieties due to their ease of staking and sprouting, and due to the fact that they have a shorter cycle, which reduces phytosanitary problems that usually occur towards the end of the growing season. This study aimed to evaluate yield components and fruit quality of ‘Floradade’ determinate growth tomato in an ecologically based production system with regard to plant density and number of stems per plant in two growing seasons, spring-summer (SS) and summer-fall (SF). Two experiments were conducted in Pelotas in the years 2010/2011 under open field conditions. Plants were trained with two or four stems and four plant densities were evaluated: 2.0; 2.5; 3.0 and 3.6 plants m-2in SS, and 1.5; 2.0; 2.5 and 3.0 plants m-2 in SF. The fruit number, fruit average weight, fruit yield, ascorbic acid content, total soluble solids and fruit pH were evaluated. The average fruit yield obtained in SF was on average 80% lower than that in SS. The increase of plant density and number of stems per plant led to an increase in fruit number and fruit yield in the two crop seasons. There was an increase in total soluble solids and reduction in the ascorbic acid content of the fruit. Two stemsper plant and plant density of 3.0 plants m-2 are recommended for the ‘Floradade’ tomato crop under an ecological production system and SS crop conditions.
650 $aTomate
700 1 $aWATTHIER, M.
700 1 $aSCHWENGBER, J. E.
700 1 $aSILVA, D. R da
700 1 $aSCHUBERT, R. N.
700 1 $aPEIL, R. M. N.
773 $tAfrican Journal of Agricultural Research$gv. 13, n. 12, p. 544-550, Mar. 2018.
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