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| 1. |  | SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M. In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines. Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018.| Biblioteca(s): Embrapa Agroenergia; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
09/02/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M. |
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Afiliação: |
WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; FERNANDO HERCOS VALICENTE, CNPMS; ROSANA FALCAO, CNPAE; MARCIO MARTINELLO SANCHES, Cenargen. |
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Título: |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018. |
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DOI: |
10.1590/S0100-204X2018000100001 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV. |
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Palavras-Chave: |
Cultura de células; Lagarta-do-cartucho; Produção in vitro; SfMNPV. |
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Thesagro: |
Baculovirus; Controle biológico; Spodoptera Frugiperda. |
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Thesaurus NAL: |
Baculoviridae; Biological control; Cell culture; Spodoptera frugiperda multiple nucleopolyhedrovirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172484/1/53n01a01.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173947/1/In-vitro-infectivity-of-Spodoptera-frugiperda.pdf
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Marc: |
LEADER 02250naa a2200313 a 4500
001 2089326
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2018000100001$2DOI
100 1 $aSIHLER, W.
245 $aIn vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines.$h[electronic resource]
260 $c2018
520 $aThe objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
650 $aBaculoviridae
650 $aBiological control
650 $aCell culture
650 $aSpodoptera frugiperda multiple nucleopolyhedrovirus
650 $aBaculovirus
650 $aControle biológico
650 $aSpodoptera Frugiperda
653 $aCultura de células
653 $aLagarta-do-cartucho
653 $aProdução in vitro
653 $aSfMNPV
700 1 $aSOUZA, M. L. de
700 1 $aVALICENTE, F. H.
700 1 $aFALCAO, R.
700 1 $aSANCHES, M. M.
773 $tPesquisa Agropecuária Brasileira, Brasília$gv. 53, n. 1, p. 1-9, 2018.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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