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| 3. |  | ROSA NETO, A. P. da; SILVA, G. M. da; MUZA, D. N.; COLPO, F. D.; SILVA, A. C. S. da. Levantamento de dados sobre produção e mercado de sementes forrageiras de clima temperado na região da Campanha do Rio Grande do Sul. In: SALÃO INTERNACIONAL DE ENSINO, EXTENSÃO E PESQUISA, 5., 2013, Bagé. Discussão, participação e cidadania: anais. Bagé: Unipampa, 2013. SIEPE.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 6. | | FRANÇA-NETO, J. B.; HENNING, A. A.; KRZYZANOWSKI, F. C.; HENNING, F. A.; LORINI, I. Adoção do tratamento industrial de sementes de soja no Brasil, safra 2014/15. Informativo ABRATES, Brasília, v. 25, n.2, set. 2015. Edição do Anais do XIX Congresso Brasileiro de Sementes, Foz do Iguaçu, set. 2015.| Biblioteca(s): Embrapa Soja. |
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| 13. | | DUARTE, R. D.; MAZZOCATO, A. C.; BRANCO, V. T. A.; SANTOS, D. da S. dos. Coletas de germoplasma para ampliação da variabilidade do Banco Ativo de Germoplasma de Forrageiras do Sul. In: SALÃO INTERNACIONAL DE ENSINO, EXTENSÃO E PESQUISA, 5., 2013, Bagé. Discussão, participação e cidadania: anais. Bagé: Unipampa, 2013. SIEPE.| Biblioteca(s): Embrapa Pecuária Sul. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
23/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
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Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
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Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
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ISSN: |
1435-8603 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Thesagro: |
Semente. |
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Categoria do assunto: |
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Marc: |
LEADER 02347naa a2200193 a 4500
001 2061470
005 2017-01-23
008 2017 bl uuuu u00u1 u #d
022 $a1435-8603
100 1 $aCALVI, G. P.
245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource]
260 $c2017
520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.
650 $aSemente
700 1 $aAUD, F. F.
700 1 $aFERRAZ, I. D. K.
700 1 $aPRITCHARD, H. W.
700 1 $aKRANNER, I.
773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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