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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/11/2015 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA, C. M. L.; VELLICCE, G. R.; GARCÍA, M. G.; PARDO, E. M.; RACEDO, J.; PERERA, M. F.; LUCÍA, A. de; GILLI, J.; BOGADO, N.; BONNECARRÈRE, V.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, L. D.; WELIN, B.; CASTAGNARO, A. P. |
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Afiliação: |
CARLA MARIA LOURDES ROCHA, ITA - NOA; GABRIEL RICARDO VELLICCE, ITA - NOA; MARÍA GABRIELA GARCÍA, ITA - NOA; ESTEBAN MARIANO PARDO, ITA - NOA; JOSEFINA RACEDO, ITA - NOA; MARÍA FRANCISCA PERERA, ITA - NOA; ADRIAN DE LUCÍA, INTA; JAVIER GILLI, INTA; NOELIA BOGADO, IPTA; VICTORIA BONNECARRÈRE, INIA; SILVIA GERMAN, INIA; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; FERNANDO LEDESMA A ,, ITA - NOA; SEBASTIÁN REZNIKOV, ITA - NOA; LEONARDO DANIEL PLOPER, ITA - NOA; BJORN WELIN, ITA - NOA; ATILIO PEDRO CASTAGNARO, ITA- NOA. |
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Título: |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, v. 18, n. 6, p. 439-444, 2015. |
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ISSN: |
0717-3458 |
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DOI: |
10.1016/j.ejbt.2015.06.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. MenosBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and pot... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Doença de planta; Ferrugem; Fungo; Phakopsora pachyrhizi; Soja. |
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Thesaurus Nal: |
Microbial growth; Plant diseases and disorders; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02845naa a2200445 a 4500
001 2028670
005 2017-07-31
008 2015 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.1016/j.ejbt.2015.06.007$2DOI
100 1 $aROCHA, C. M. L.
245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource]
260 $c2015
520 $aBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
650 $aMicrobial growth
650 $aPlant diseases and disorders
650 $aSoybean rust
650 $aDoença de planta
650 $aFerrugem
650 $aFungo
650 $aPhakopsora pachyrhizi
650 $aSoja
653 $aFerrugem asiática da soja
700 1 $aVELLICCE, G. R.
700 1 $aGARCÍA, M. G.
700 1 $aPARDO, E. M.
700 1 $aRACEDO, J.
700 1 $aPERERA, M. F.
700 1 $aLUCÍA, A. de
700 1 $aGILLI, J.
700 1 $aBOGADO, N.
700 1 $aBONNECARRÈRE, V.
700 1 $aGERMAN, S.
700 1 $aMARCELINO, F.
700 1 $aLEDESMA, F.
700 1 $aREZNIKOV, S.
700 1 $aPLOPER, L. D.
700 1 $aWELIN, B.
700 1 $aCASTAGNARO, A. P.
773 $tElectronic Journal of Biotechnology$gv. 18, n. 6, p. 439-444, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
Volume |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
16/09/2015 |
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Data da última atualização: |
21/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOARES, A. P. G.; GUILLIN, E. A.; BORGES, L. L.; SILVA, A. C. T. da; ALMEIDA, A. M. R. de; GRIJALBA, P. E.; GOTTLIEB, A. M.; BLUHM, B. H.; OLIVEIRA, L. O. de. |
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Afiliação: |
ANA PAULA GOMES SOARES, UFV; EDUARDO A. GUILLIN, INTA; LEANDRO LUIZ BORGES, UFV; Amanda C. T. da Silva, UFV; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSO; PABLO E. GRIJALBA, Universidad de Buenos Aires; ALEXANDRA M. GOTTLIEB, CONICET; BURTON H. BLUHM, University of Arkansas; LUIZ ORLANDO DE OLIVEIRA, UFV. |
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Título: |
More Cercospora species infect soybeans across the Americas than meets the eye. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 8, e0133495, Aug. 2015. |
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DOI: |
10.1371/journal.pone.0133495 |
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Idioma: |
Inglês |
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Conteúdo: |
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ~80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. MenosDiseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ~80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monoph... Mostrar Tudo |
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Thesagro: |
Doença de planta; Fungo; Soja. |
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Thesaurus NAL: |
Plant diseases and disorders; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1024076/1/MoreCercosporaSpeciesInfectSoybeans.pdf
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Marc: |
LEADER 02948naa a2200289 a 4500
001 2024076
005 2017-06-21
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0133495$2DOI
100 1 $aSOARES, A. P. G.
245 $aMore Cercospora species infect soybeans across the Americas than meets the eye.$h[electronic resource]
260 $c2015
520 $aDiseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ~80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.
650 $aPlant diseases and disorders
650 $aSoybeans
650 $aDoença de planta
650 $aFungo
650 $aSoja
700 1 $aGUILLIN, E. A.
700 1 $aBORGES, L. L.
700 1 $aSILVA, A. C. T. da
700 1 $aALMEIDA, A. M. R. de
700 1 $aGRIJALBA, P. E.
700 1 $aGOTTLIEB, A. M.
700 1 $aBLUHM, B. H.
700 1 $aOLIVEIRA, L. O. de
773 $tPlos One$gv. 10, n. 8, e0133495, Aug. 2015.
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