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| 1. |  | OLIVEIRA, M. C. de S.; OLIVEIRA-SIQUEIRA, T. C. G.; NEO, T. A.; BRITO, L. G.; DAVID, E. B.; REGITANO, L. C. de A. Atividade proteolítica dos produtos de excreção/secreção de larvas de Cochliomyia homonivorax. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 14.; SIMPÓSIO LATINO-AMERICANO DE RICKETTSIOSES, 2., 2006, Ribeirão Preto. Anais... Ribeirão Preto: CBPV, 2006. p. 194.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Rondônia. |
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| 2. | | OLIVEIRA, M. C. de S.; OLIVEIRA-SIQUEIRA, T. C. G.; REGITANO, L. C. de A.; ALENCAR, M. M. de; NÉO, T. A.; SILVA, A. M.; OLIVEIRA, H. N. Detection of Babesia bigemina in cattle of different genetic groups and in Rhipicephalus (Boophilus) microplus tick. Veterinary Parasitology, v. 155, n. 3-4, p. 281- 286, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | BILHASSI, T. B.; OLIVEIRA, H. N.; IBELLI, A. M. G.; GIGLIOTI, R.; REGITANO, L. C. de A.; OLIVEIRA-SIQUEIRA, T. C. G.; BRESSANI, F. A.; MALAGO JUNIOR, W.; RESENDE, F. D.; OLIVEIRA, M. C. de S. Quantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil. Ticks and Tick-borne Diseases, v. 5, P. 234-238, 2014| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 4 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/12/2014 |
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Data da última atualização: |
01/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BILHASSI, T. B.; OLIVEIRA, H. N.; IBELLI, A. M. G.; GIGLIOTI, R.; REGITANO, L. C. de A.; OLIVEIRA-SIQUEIRA, T. C. G.; BRESSANI, F. A.; MALAGO JUNIOR, W.; RESENDE, F. D.; OLIVEIRA, M. C. de S. |
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Afiliação: |
TALITA B. BILHASSI, UNESP/JABOTICABAL; HENRIQUE N. OLIVEIRA, UNESP/JABOTICABAL; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; RODRIGO GIGLIOTI, UNESP/JABOTICABAL; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; Teresa C.G. Oliveira-Sequeira, UNESP/BOTUCATU; FLAVIA ALINE BRESSANI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; FLAVIO D. RESENDE, APTA/POLO REGIONAL ALTA MOGIANA/COLINA; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Quantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 5, P. 234-238, 2014 |
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Idioma: |
Português |
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Conteúdo: |
infection in beef cattle raised in areas endemic for babesiosis in Brazil, where the animals were continuously exposed to ticks (Rhipicephalus microplus). This is the first report in which qPCR was used to quantify and compare B. bovis DNA in blood of different cattle breeds. Blood samples were collected from 150 animals (75 cows and 75 calves) of the Angus and Nelore breeds and the first generation of an Angus and Nelore cross (AxN). Blood samples from the jugular vein were used for DNA extraction and determination of packed cell volume (PCV), while samples from peripheral veins were used for microscopic parasite detection. Although no piroplasms of B. bovis were found in blood smears, DNA amplification using qPCR revealed that all of the 150 animals, except two calves and one cow, were positive. The number of copies of B. bovis DNA was higher
(p < 0.05) in the Angus than in the Nelore and AxN animals, for both calves and cows, but no significant difference was found between the Nelore and AxN groups. These results suggest that a heterotic effect was present, since the results from the crossbred animals significantly deviated from the mean of the two parental groups, while closely approaching that of the Nelore group. In the Nelore and AxN groups, calves showed higher infection levels than cows (p < 0.05), while for the Angus group the difference was found to be non-significant. Within each animal age group, the breed groups with higher infection levels were those with lower PCV values. However, within each breed group, no significant correlations were found between the number of DNA copies and PCV according to animal age. The qPCR method applied here allowed the ob ervation that although there are no differences in the prevalence of infection among breed groups, Nelore and AxN cattle are able to maintain infection by B. bovis at lower levels than the Angus cattle. Menosinfection in beef cattle raised in areas endemic for babesiosis in Brazil, where the animals were continuously exposed to ticks (Rhipicephalus microplus). This is the first report in which qPCR was used to quantify and compare B. bovis DNA in blood of different cattle breeds. Blood samples were collected from 150 animals (75 cows and 75 calves) of the Angus and Nelore breeds and the first generation of an Angus and Nelore cross (AxN). Blood samples from the jugular vein were used for DNA extraction and determination of packed cell volume (PCV), while samples from peripheral veins were used for microscopic parasite detection. Although no piroplasms of B. bovis were found in blood smears, DNA amplification using qPCR revealed that all of the 150 animals, except two calves and one cow, were positive. The number of copies of B. bovis DNA was higher
(p < 0.05) in the Angus than in the Nelore and AxN animals, for both calves and cows, but no significant difference was found between the Nelore and AxN groups. These results suggest that a heterotic effect was present, since the results from the crossbred animals significantly deviated from the mean of the two parental groups, while closely approaching that of the Nelore group. In the Nelore and AxN groups, calves showed higher infection levels than cows (p < 0.05), while for the Angus group the difference was found to be non-significant. Within each animal age group, the breed groups with higher infection levels were those with low... Mostrar Tudo |
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Palavras-Chave: |
B bovis; QPCR. |
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Thesaurus NAL: |
babesiosis; beef cattle. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02680naa a2200277 a 4500
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008 2014 bl --- 0-- u #d
100 1 $aBILHASSI, T. B.
245 $aQuantitative study of Babesia bovis infection in beef cattle from São Paulo state, Brazil.
260 $c2014
520 $ainfection in beef cattle raised in areas endemic for babesiosis in Brazil, where the animals were continuously exposed to ticks (Rhipicephalus microplus). This is the first report in which qPCR was used to quantify and compare B. bovis DNA in blood of different cattle breeds. Blood samples were collected from 150 animals (75 cows and 75 calves) of the Angus and Nelore breeds and the first generation of an Angus and Nelore cross (AxN). Blood samples from the jugular vein were used for DNA extraction and determination of packed cell volume (PCV), while samples from peripheral veins were used for microscopic parasite detection. Although no piroplasms of B. bovis were found in blood smears, DNA amplification using qPCR revealed that all of the 150 animals, except two calves and one cow, were positive. The number of copies of B. bovis DNA was higher (p < 0.05) in the Angus than in the Nelore and AxN animals, for both calves and cows, but no significant difference was found between the Nelore and AxN groups. These results suggest that a heterotic effect was present, since the results from the crossbred animals significantly deviated from the mean of the two parental groups, while closely approaching that of the Nelore group. In the Nelore and AxN groups, calves showed higher infection levels than cows (p < 0.05), while for the Angus group the difference was found to be non-significant. Within each animal age group, the breed groups with higher infection levels were those with lower PCV values. However, within each breed group, no significant correlations were found between the number of DNA copies and PCV according to animal age. The qPCR method applied here allowed the ob ervation that although there are no differences in the prevalence of infection among breed groups, Nelore and AxN cattle are able to maintain infection by B. bovis at lower levels than the Angus cattle.
650 $ababesiosis
650 $abeef cattle
653 $aB bovis
653 $aQPCR
700 1 $aOLIVEIRA, H. N.
700 1 $aIBELLI, A. M. G.
700 1 $aGIGLIOTI, R.
700 1 $aREGITANO, L. C. de A.
700 1 $aOLIVEIRA-SIQUEIRA, T. C. G.
700 1 $aBRESSANI, F. A.
700 1 $aMALAGO JUNIOR, W.
700 1 $aRESENDE, F. D.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 5, P. 234-238, 2014
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