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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/05/2014 |
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Data da última atualização: |
24/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; SILVA, R. B. da. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; ROSANE BEZERRA DA SILVA, BOLSISTA. |
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Título: |
fAFLP analysis of Brazilian Bacillus thuringiensis isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
SpringerPlus, v. 3, n. 256, 2014. |
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DOI: |
10.1186/2193-1801-3-256 |
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Idioma: |
Inglês |
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Conteúdo: |
A total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors. MenosA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest s... Mostrar Tudo |
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Palavras-Chave: |
Bacillus thruringiensis; Diversidade genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02280naa a2200169 a 4500
001 1987279
005 2017-05-24
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/2193-1801-3-256$2DOI
100 1 $aVALICENTE, F. H.
245 $afAFLP analysis of Brazilian Bacillus thuringiensis isolates.$h[electronic resource]
260 $c2014
520 $aA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors.
653 $aBacillus thruringiensis
653 $aDiversidade genética
700 1 $aSILVA, R. B. da
773 $tSpringerPlus$gv. 3, n. 256, 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 14 | |
| 1. |  | BARRABIN, J. S.; SCHULZ, D. F.; GODOY, R. L. de O.; BORGUINI, R. G.; ROSA, J. S. da. Análise preliminar de aflatoxinas por UPLC acoplado a espectrômetro de massas Q-TOF. Higiene Alimentar, São Paulo, v. 25, n. 194/195, p. 705-706, mar./abr. 2011. Edição dos Trabalhos apresentados no V Congresso Latino Americano e XI Congresso Brasileiro de Higienistas de Alimentos, I Encontro Nacional de Vigilância de Zoonoses e Agravos Causados por Animais de Interesse à Saúde Pública, III Encontro do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Salvador, abr. 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 2. | | GOUVÊA, A. C. M. S.; ARAUJO, M. C. P. de; SCHULZ, D. F.; PACHECO, S.; GODOY, R. L. de O.; CABRAL, L. M. C. Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried açaí (Euterpe oleraceae Mart.) by HPLC. Ciência e Tecnologia de Alimentos, Campinas, v. 32, n. 1, p. 1-4, jan./mar. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 3. | | ROSA, J. S. da; OIANO NETO, J.; FURTADO, A. A. L.; GODOY, R. L. de O.; BORGUINI, R. G.; SCHULZ, D. F.; BARRABIN, J. S.; IGLESIAS, A. N.; REZENDE, C. M. de. Comparison of analytical methods for determination of limonoids in tangerine juice using HPLC-PDA and ULC-ESI-Q-TOF. In: CONGRESSO BRASILEIRO DE ESPECTROMIA DE MASSAS, 4., 2011, Campinas. Anais... Campinas: BrMASS, 2011| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | POMBO, C. R.; MÁRSICO, E. T.; SCHULZ, D. F.; GODOY, R. L. O.; PACHECO, S. Desenvolvimento de novo método de análise de histamina, putrescina e cadaverina por CLAE utilizando derivatização com 6-aminoquinolil-n-hidroxisuccinimidil carbamato (AQC). In: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 3., 2010, Florianópolis. Rompendo barreiras: anais. Florianópolis: SBCTA-RS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 8. | | GODOY, R. L. O.; SCHULZ, D. F.; ROSA, J. S.; OIANO-NETO, J.; NARAIN, N.; SANTIAGO, M. C. P. A.; PACHECO, P.; NASCIMENTO, L. S. M.; REZENDE, C. M.; DA CUNHA PINTO, A. Development of an LC-MS-MS ESI-qTOF method for determination of tetracyclines residues in bovine milk. In: INTERNATIONAL CIGR WORKSHOP ON FOOD SAFETY, 1., 2011, Dijon. Advances and trends: book of abstracts. Dijon: AgroSup Dijon, 2011. p. 76.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | ROSA, J. S.; SCHULZ, D. F.; GODOY, R. L. O.; OIANO-NETO, J.; BOBEDA, C. R. R.; SANTIAGO, M. C. P. A.; PACHECO, S.; NASCIMENTO, L. S. M.; DA CUNHA PINTO, A.; REZENDE, C. M. Development of an LC-MS-MS ESI-QTOF method for melamine determination in milk. In: INTERNATIONAL CIGR WORKSHOP ON FOOD SAFETY, 1., 2011, Dijon. Advances and trends: book of abstracts. Dijon: AgroSup Dijon, 2011. p. 75.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 10. | | SCHULZ, D. F.; ROSA, J. S.; RAICES, R. S. L.; GODOY, R. L. O.; OIANO-NETO, J.; SANTIAGO, M. C. P. A.; PACHECO, S.; BARBARIN, J. S.; MATTOS, L. S.; REZENDE, C. M.; DA CUNHA PINTO, A. Development of an LC-MS-MS ESI-qTOF method for the determination of chloramphenicol. In: INTERNATIONAL CIGR WORKSHOP ON FOOD SAFETY, 1., 2011, Dijon. Advances and trends: book of abstracts. Dijon: AgroSup Dijon, 2011. p. 73.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | GOUVÊA, A. C. M. S.; SCHULZ, D. F.; SANTIAGO, M. C. P. de A.; GODOY, R. L. de O.; PACHECO, S.; ROSA, J. S. Identification of jambo (Syzygium malaccense L.) major anthocyanin by LC-MS-MS. In: ENCONTRO NACIONAL DE CROMATOGRAFIA, 7., 2012, Porto. Posters... Porto: Sociedade Portuguesa de Química, 2012. p. 168.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | DIAS, S. da S.; GODOY, R. L. de O.; PACHECO, S.; COELHO, M. S.; SCHULZ, D. F.; REIS, R. C. S.; BOBEDA, C. R. R. Obtenção de padrão analítico de retinol a partir de fígado bovino por cromatografia líquida de alta eficiência. Revista Semioses, v. 11, n. 3, p. 38-42, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos. |
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| 13. | | SCHULZ, D. F.; PACHECO, S.; ROSA, J. S.; PINTO, A. C.; GODOY, R. L. O. Preparação de padrões cromatográficos de betacaroteno e zeaxantina, com confirmação estrutural por ESI/QqTOF. In: SIMPÓSIO BRASILEIRO DE CROMATOGRAFIA E TÉCNICAS AFINS, 4., 2010, Campos do Jordão. Livro de resumos. São Carlos, SP: Instituto Internacional de Cromatografia, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 14. | | BARRABIN, J. S.; BORGUINI, R. G.; GODOY, R. L. O.; ROSA, J. S. da; SCHULZ, D. F. Preparation of calibration curves for UPLC-ESI-QTOF-MS determination of aflatoxinas B1, B2, G1, and G2. In: ISM CONFERENCE, 2011, Mendoza. Strategies to reduce the impact of mycotoxins in Latin America in a global context: book of abstracts. Río Cuarto: Universidad Nacional de Río Cuarto, 2011. p. 233.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 14 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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