Data/hora: 27/07/2024 06:59:59

Biblioteca(s): 	Embrapa Suínos e Aves.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	05/11/2020	
Data da última atualização: 	05/11/2020	
Autoria: 	SOARES, S. L.; ANCIUTIA, A. N.; DIAS, L.; CORCINI, C. D.; VARELA JÚNIOR, A. S.; KOMNINOU, E. R.; TEBALDI, M. L.; MARQUES, M. G.; FONSECA, F. M. da; LUCIA JUNIOR, T.	
Afiliação: 	SARA L. SOARES, UFPel; ANDREIA N. ANCIUTI, UFPel; LUCIA DIAS, UFPel; CARINE DAHL CORCINI, UFPel; ANTÔNIO S. VARELA JÚNIOR, FURG; ELIZA R. KOMNINOU, UFPel; MARLI LUIZA TEBALDI, UNIFEI; MARIANA GROKE MARQUES, CNPSA; FRANCISCO NOE DA FONSECA, CNPSA; THOMAZ LUCIA JUNIOR, UFPel.	
Título: 	Safety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Toxicology in Vitro, v. 65, n. 104766, 2020.	
DOI: 	https://doi.org/10.1016/j.tiv.2020.104766	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 &#956;g/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P > .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P > .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P > .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P > .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders.	
Thesagro: 	Criopreservação; Espermatozóide; Javali; Reprodução Animal.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (21964-1) - UPC - AP - DD -  -  -  - DIS